Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Kayeong Lim"'
Autor:
Jaesuk Lee, Kayeong Lim, Annie Kim, Young Geun Mok, Eugene Chung, Sung-Ik Cho, Ji Min Lee, Jin-Soo Kim
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-11 (2023)
Cas9 nickases (nCas9s) produce nicks or single-strand breaks in the DNA. Here the authors analyse the on- and off-target nicks generated by these nickases, and show that nCas9 (H840A) but not nCas9 (D10A) can cleave both strands and produce unwanted
Externí odkaz:
https://doaj.org/article/d4e6a4c2bb8349c89d41ddb7631ab4af
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-10 (2022)
Monomeric DdCBEs (mDdCBEs), enabling mitochondrial DNA editing with high efficiencies of up to 50%, when transiently expressed in cultured human cells and nearly homoplasmic C-to-T editing via AAV.
Externí odkaz:
https://doaj.org/article/217dabfa7f04465aa29214433432d07a
Autor:
Daniel Whisenant, Kayeong Lim, Gwladys Revêchon, Haidong Yao, Martin O. Bergo, Piotr Machtel, Jin-Soo Kim, Maria Eriksson
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)
Base editing to treat diseases is progressing but tissue delivery and progenitor cells correction are challenging. Here, the authors show sustained effects and propagation of mutation-corrected progenitors by transient adenine base editor expression,
Externí odkaz:
https://doaj.org/article/5033953156d74b62be0f6190506dc67f
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-10 (2022)
Base editing in nuclear DNA and mitochondrial DNA (mtDNA) is broadly useful for biomedical research, medicine, and biotechnology. Here the authors present zinc finger deaminases which catalyze targeted C-to-T base conversions without inducing unwante
Externí odkaz:
https://doaj.org/article/b953e7a175d541f19ce765ed3b4a5318
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
Cas12a-linked base editors can broaden the targeting scope of programmable cytidine deaminases. Here the authors assess their target specificity in an in vitro genome-wide assay.
Externí odkaz:
https://doaj.org/article/b0a64a1338ff4e0885c245ba597b7e03
Autor:
Gue-Ho Hwang, Jihyeon Yu, Soyeon Yang, Woo Jae Son, Kayeong Lim, Heon Seok Kim, Jin-Soo Kim, Sangsu Bae
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 18, Iss , Pp 1686-1694 (2020)
CRISPR-Cas9 induces DNA cleavages at desired target sites in a guide RNA-dependent manner; DNA editing occurs through the resulting activity of DNA repair processes including non-homologous end joining (NHEJ), which is dominant in mammalian cells. NH
Externí odkaz:
https://doaj.org/article/dbe7d96088ba45f6aa3e68441b0fab96
Autor:
Eunjin Cho, Seongmin Cheon, Mina Ding, Kayeong Lim, Sang-Wook Park, Chungoo Park, Tae-Hoon Lee
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 12, p 6421 (2022)
Osteoclasts are derived from hematopoietic stem cells. Monocyte preosteoclasts obtain resorbing activity via cell–cell fusion to generate multinucleated cells. However, the mechanisms and molecules involved in the fusion process are poorly understo
Externí odkaz:
https://doaj.org/article/687b9e9035154e97a0dd29b25a2c9296
Autor:
Gue-Ho Hwang, Jeongbin Park, Kayeong Lim, Sunghyun Kim, Jihyeon Yu, Eunchong Yu, Sang-Tae Kim, Roland Eils, Jin-Soo Kim, Sangsu Bae
Publikováno v:
BMC Bioinformatics, Vol 19, Iss 1, Pp 1-7 (2018)
Abstract Background As a result of its simplicity and high efficiency, the CRISPR-Cas system has been widely used as a genome editing tool. Recently, CRISPR base editors, which consist of deactivated Cas9 (dCas9) or Cas9 nickase (nCas9) linked with a
Externí odkaz:
https://doaj.org/article/6f3d4be073304c12bc5c677fb04bf7f0
Autor:
Jaesuk Lee, Kayeong Lim, Annie Kim, Young Geun Mok, Eugene Chung, Sung-Ik Cho, Ji Min Lee, Jin-Soo Kim
Unlike CRISPR-Cas9 nucleases, which yield DNA double-strand breaks (DSBs), Cas9 nickases (nCas9s), which are created by replacing key catalytic amino-acid residues in one of the two nuclease domains of S. pyogenesis Cas9 (SpCas9), produce nicks or si
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d074bb2c224754fe6dbb1b86508a0a41
https://doi.org/10.21203/rs.3.rs-2236723/v1
https://doi.org/10.21203/rs.3.rs-2236723/v1
Autor:
Sangsu Bae, Omer Habib, Sung Ah Hong, Gue Ho Hwang, You Kyeong Jeong, Jin-Soo Kim, Kayeong Lim
Publikováno v:
Nucleic Acids Research
Prime editing technology is capable of generating targeted insertions, deletions, and base conversions. However, the process of designing prime editing guide RNAs (pegRNAs), which contain a primer binding site and a reverse-transcription template at