Analysis of Salmonella enterica Serovar Typhi by Outer Membrane Protein (OMP) Profiling, Random Amplification of Polymorphic DNA (RAPD) and Pulsed Field Gel Electrophoresis (PFGE)

Autor: Kavaratty Raju Mani, Ajay Kumar Tahlan, Yashwant Kumar

Publikováno v: Tropical Life Sciences Research

A number of countries, including developed countries, still have typhoid fever as a major problem resulting in frequent outbreaks. The importance of controlling spread of typhoid fever is well known and necessitates periodic studies to delineate epid

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eebb7278c64172aee5dde63d3888a212
https://doi.org/10.21315/tlsr2019.30.1.4

Standardization of process for increased production of pure and potent tetanus toxin

Autor: Chellamani Muniandi, Kavaratty Raju Mani, Subashkumar Rathinasamy, Premkumar Lakshmanan

Publikováno v: Journal of Microbiology and Infectious Diseases, Vol 3, Iss 3, Pp 133-139 (2013)
Volume: 3, Issue: 03 133-140
Journal of Microbiology and Infectious Diseases

Objectives: The aim of the study was to increase the yield of tetanus toxin in short time fermentor cultivation and also to produce pure and potent tetanus toxin replacing initial nitrogen source (N.Z Case) with papain digest broth in the modified Mu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2f57a80551cd212eb30efc058209463
https://doi.org/10.5799/ahinjs.02.2013.03.0096

Process standardization for optimal virus recovery and removal of substrate DNA and bovine serum proteins in Vero cell-derived rabies vaccine

Autor: Kavaratty Raju Mani, Ananda Arone Prem Kumar, Yarlagadda Udaya Bhaskara Rao, K. Swaminathan, Arokiaswami Leo William Joseph

Publikováno v: Journal of Bioscience and Bioengineering. 94:375-383

Purification of a rabies vaccine by a single zonal centrifugation run was replaced by two runs with optimal standardization of the sucrose density gradient. As a result, significant reductions in the levels of substrate DNA and bovine serum protein i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04c77578cb4c83c20136f3f080cdcce5
https://doi.org/10.1016/s1389-1723(02)80212-2

Re-emergence of susceptibility to conventionally used drugs among strains of Salmonella Typhi in central west India

Autor: Anshu Sharma, Yashwant Kumar, Kavaratty Raju Mani

Publikováno v: Journal of infection in developing countries. 5(3)

Introduction: Typhoid fever (enteric fever) is a global health problem causing high morbidity and mortality, especially in endemic areas such as India. The problem is exacerbated as the causative agent, Salmonella enterica subspecies enterica serovar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c667d5829ca7c018e048ea27c978d064
https://pubmed.ncbi.nlm.nih.gov/21444993

Purification, potency and immunogenicity analysis of Vero cell culture-derived rabies vaccine: a comparative study of single-step column chromatography and zonal centrifuge purification

Autor: Chitrambalam Palaniappan, Lakshman Narasingha Rao Bhau, Ananda Arone Prem Kumar, Kavaratty Raju Mani, K. Swaminathan

Publikováno v: Microbes and infection. 7(9-10)

Continuous Vero cell lines are more suitable for large-scale production of rabies vaccine. The purification of Vero cell-derived rabies vaccine is critical because of the residual cellular DNA and serum proteins. The perfection of techniques using co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0ba0e4a0ad3b240fc3ee3347bb00609
https://pubmed.ncbi.nlm.nih.gov/16046167