Data from CD137/OX40 Bispecific Antibody Induces Potent Antitumor Activity that Is Dependent on Target Coengagement

Autor: Neil Brewis, Mihriban Tuna, Michelle Morrow, Francisca Wollerton, Katy L. Everett, Sandra Uhlenbroich, Leonor Rodrigues, John Pravin, Miguel Gaspar

Following the success of immune checkpoint blockade therapy against cancer, agonistic antibodies targeting T-cell costimulatory pathways are in clinical trials. The TNF superfamily of receptors (TNFRSF) members CD137 and OX40 are costimulatory recept

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::236ce70bc6065bd5ca45e829eec35a48
https://doi.org/10.1158/2326-6066.c.6550416

An improved targeted cAMP sensor to study the regulation of adenylyl cyclase 8 by Ca2+ entry through voltage-gated channels.

Autor: Katy L Everett, Dermot M F Cooper

Publikováno v: PLoS ONE, Vol 8, Iss 9, p e75942 (2013)

Here we describe an improved sensor with reduced pH sensitivity tethered to adenylyl cyclase (AC) 8. The sensor was used to study cAMP dynamics in the AC8 microdomain of MIN6 cells, a pancreatic β-cell line. In these cells, AC8 was activated by Ca(2

Externí odkaz: https://doaj.org/article/f7d48f3b4ca34225bdabad5d02517c5e

Muscarinic receptors stimulate AC2 by novel phosphorylation sites, whereas Gβγ subunits exert opposing effects depending on the G-protein source

Autor: Michelle L. Halls, Dermot M.F. Cooper, Jia Xiao Shen, Sebastian Wachten, Katy L. Everett

Publikováno v: Biochemical Journal

Direct phosphorylation of AC2 (adenylyl cyclase 2) by PKC (protein kinase C) affords an opportunity for AC2 to integrate signals from non-canonical pathways to produce the second messenger, cyclic AMP. The present study shows that stimulation of AC2

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4dd11b40a8af35fdf66cda97c3392abb
https://doi.org/10.1042/bj20120279

Membrane Environment Exerts an Important Influence on Rac-Mediated Activation of Phospholipase Cγ2

Autor: Paul M. W. French, Petra Vatter, Hugh B. Manning, Anja Buehler, Christopher Dunsby, Michael Retlich, Tom D. Bunney, Katy L. Everett, Matilda Katan, Anca Margineanu, Peter Gierschik, Claudia Walliser, Rhona W. Baxendale, Mark A. A. Neil

Publikováno v: Molecular and Cellular Biology. 31:1240-1251

We performed analyses of the molecular mechanisms involved in the regulation of phospholipase C gamma 2 (PLC gamma 2). We identified several regions in the PLC gamma-specific array, gamma SA, that contribute to autoinhibition in the basal state by oc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fec404f3b5e39c2b98cf995c6b4375a
https://doi.org/10.1128/mcb.01408-10

ADCY5 couples glucose to insulin secretion in human islets

Autor: Stephen J. Hughes, Guy A. Rutter, Lorella Marselli, Vanessa Lavallard, David J. Hodson, Daliang Li, Piero Marchetti, Ryan K. Mitchell, Katy L. Everett, Francesca Semplici, Timothy J. Pullen, Wen Hong Li, Marco Bugliani, Silvia Gimeno Brias, Dermot M.F. Cooper, Lorenzo Piemonti, Paul Johnson, Domenico Bosco, A. M. James Shapiro

Publikováno v: Diabetes
Diabetes, Vol. 63, No 9 (2014) pp. 3009-21
Hodson, D J, Mitchell, R K, Marselli, L, Pullen, T J, Gimeno Brias, S, Semplici, F, Everett, K L, Cooper, D M F, Bugliani, M, Marchetti, P, Lavallard, V, Bosco, D, Piemonti, L, Johnson, P R, Hughes, S J, Li, D, Li, W-H, Shapiro, A M J & Rutter, G A 2014, ' ADCY5 couples glucose to insulin secretion in human islets ', Diabetes, vol. 63, no. 9, pp. 3009-3021 . https://doi.org/10.2337/db13-1607

Single nucleotide polymorphisms (SNPs) within the ADCY5 gene, encoding adenylate cyclase 5, are associated with elevated fasting glucose and increased type 2 diabetes (T2D) risk. Despite this, the mechanisms underlying the effects of these polymorphi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10e1e868ddcf2a6b7c6b12310e058850
https://pubmed.ncbi.nlm.nih.gov/24740569