Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Katsumi Morimatsu"'
Publikováno v:
Journal of Biological Chemistry. 287:35621-35630
The repair of single-stranded gaps in duplex DNA by homologous recombination requires the proteins of the RecF pathway. The assembly of RecA protein onto gapped DNA (gDNA) that is complexed with the single-stranded DNA-binding protein is accelerated
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::aab5ff2414759e37f03d2e114082d0a3
https://doi.org/10.1074/jbc.m112.397034
https://doi.org/10.1074/jbc.m112.397034
Publikováno v:
Genes & Development. 23:1234-1245
The RecF pathway of Escherichia coli is important for recombinational repair of DNA breaks and gaps. Here `we reconstitute in vitro a seven-protein reaction that recapitulates early steps of dsDNA break repair using purified RecA, RecF, RecO, RecR, R
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::267525c2dbe4d720e51c420af364231e
https://doi.org/10.1101/gad.1780709
https://doi.org/10.1101/gad.1780709
Autor:
Katsumi Morimatsu, Masayuki Takahashi
Publikováno v:
Analytical Biochemistry. 358:192-198
We have developed a simple measuring system for fluorescence-detected linear dichroism and applied it to the structural analysis of the RecA-DNA complex filaments, which are intermediates of the homologous recombination reaction. Taking advantage of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdfde3c9eb506044b601b9be76164874
https://doi.org/10.1016/j.ab.2006.08.034
https://doi.org/10.1016/j.ab.2006.08.034
Publikováno v:
Molecular Cell. 11(5):1337-1347
Genetic evidence suggests that the RecF, RecO, and RecR (RecFOR) proteins participate in a common step of DNA recombination and repair, yet the biochemical event requiring collaboration of all three proteins is unknown. Here, we show that the concert
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e3453179dac05864e4bda8c4b0d11d1
Publikováno v:
Genes to Cells. 7:1125-1134
Background: Human Rad51 protein (HsRad51) is a homologue of Escherichia coli RecA protein, and involved in homologous recombination. These eukaryotic and bacterial proteins catalyse strand exchange between two homologous DNA molecules, each forming a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fb815024c5c1c3a4d671d1b38f804c49
https://doi.org/10.1046/j.1365-2443.2002.00588.x
https://doi.org/10.1046/j.1365-2443.2002.00588.x
Publikováno v:
Proceedings of the National Academy of Sciences. 99:11688-11693
Linear dichroism (LD) polarized-light spectroscopy is used to determine the arrangement of RecA in its large filamentous complex with DNA, active in homologous recombination. Angular orientation data for two tryptophan and seven tyrosine residues, de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d32651846f16c0477454017efba2906
https://doi.org/10.1073/pnas.142404499
https://doi.org/10.1073/pnas.142404499
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, vol 111, iss 48
Recombinational DNA repair by the RecF pathway of Escherichia coli requires the coordinated activities of RecA, RecFOR, RecQ, RecJ, and single-strand DNA binding (SSB) proteins. These proteins facilitate formation of homologously paired joint molecul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ceb950c9a885f25de56de928a95ebda
https://europepmc.org/articles/PMC4260596/
https://europepmc.org/articles/PMC4260596/
Publikováno v:
Journal of Molecular Biology. 306:189-199
We investigated the structure of the active RecA-DNA complex by analyzing the environment of tyrosine residue 65, which is on the DNA-binding surface of the protein. We prepared a modified RecA protein in which the tyrosine residue was replaced by tr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::29da805ec2053ffe65611ebaade7d97b
https://doi.org/10.1006/jmbi.2000.4382
https://doi.org/10.1006/jmbi.2000.4382
Publikováno v:
Genes to Cells. 1:1057-1068
BACKGROUND: The RAD51 gene of Saccharomyces cerevisiae is homologous to the Escherichia coli recA gene and plays a key role in genetic recombination and DNA double-strand break repair. To construct an improved experimental system of homologous recomb
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::224c7cb310688a19b772af1bb1dbe30b
https://doi.org/10.1046/j.1365-2443.1996.d01-224.x
https://doi.org/10.1046/j.1365-2443.1996.d01-224.x
Publikováno v:
European Journal of Biochemistry. 228:779-785
To obtain structural insight on the interaction of the RecA protein with nucleotide cofactors (ATP and ADP) and DNA, we have made two engineered RecA proteins, in which either Tyr103 or Tyr264 was replaced with tryptophan. The fluorescence of tryptop
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8de7e88174e6b8a744be8f394f8b0b5c
https://doi.org/10.1111/j.1432-1033.1995.0779m.x
https://doi.org/10.1111/j.1432-1033.1995.0779m.x