Výsledky vyhledávání - "Katsuhiko S, Murakami"

Akademický článek

Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity

Autor: Lidiya Lisitskaya, Yeonoh Shin, Aleksei Agapov, Anna Olina, Ekaterina Kropocheva, Sergei Ryazansky, Alexei A. Aravin, Daria Esyunina, Katsuhiko S. Murakami, Andrey Kulbachinskiy

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)

The authors describe programmable Argonaute nucleases that use small DNA guides to recognize and cleave RNA targets and can sense mismatches and modifications in RNA targets, making them potential tools for RNA biotechnology.

Externí odkaz: https://doaj.org/article/922fcc64ae404c05b9dbd574e4b247a5

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Akademický článek

Identification of SARS-CoV-2 inhibitors targeting Mpro and PLpro using in-cell-protease assay

Autor: Anoop Narayanan, Manju Narwal, Sydney A. Majowicz, Carmine Varricchio, Shay A. Toner, Carlo Ballatore, Andrea Brancale, Katsuhiko S. Murakami, Joyce Jose

Publikováno v: Communications Biology, Vol 5, Iss 1, Pp 1-17 (2022)

The development of new drugs to treat COVID-19 continues to be of critical importance. This paper describes a new in-cell assay to screen for inhibitors of SARSCoV-2 in live cells. 8 new compounds are reported along with a crystal structure of a key

Externí odkaz: https://doaj.org/article/149ed658ae4b462fb62bb45b9340fa58

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Akademický článek

The mechanism of the nucleo-sugar selection by multi-subunit RNA polymerases

Autor: Janne J. Mäkinen, Yeonoh Shin, Eeva Vieras, Pasi Virta, Mikko Metsä-Ketelä, Katsuhiko S. Murakami, Georgiy A. Belogurov

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)

RNA and DNA polymerases need to discriminate efficiently against closely related nucleotide triphosphate substrates. Here, the authors show that a conserved Arg residue is the major determinant of selectivity against deoxyribonucleoside substrates by

Externí odkaz: https://doaj.org/article/1a267789452f415e8760bfc2fe901636

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Akademický článek

Structural basis of ribosomal RNA transcription regulation

Autor: Yeonoh Shin, M. Zuhaib Qayyum, Danil Pupov, Daria Esyunina, Andrey Kulbachinskiy, Katsuhiko S. Murakami

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)

Ribosomal RNA (rRNA) expression is regulated at the initiation stage of RNA synthesis. Here, the authors report cryo-EM structures of E. coli RNA polymerase and rRNA promoter complex with DksA/ppGpp on the way to open complex formation, identifying k

Externí odkaz: https://doaj.org/article/c99ba2ef42594e0db946ed135fb5c245

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Akademický článek

Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)

The bacterial helicase-like transcription factor HelD associates with the RNA polymerase (RNAP) and recycles stalled transcription complexes. Here, the authors present the cryo-EM structures of three Mycobacterium smegmatis HelD bound RNAP complexes

Externí odkaz: https://doaj.org/article/d65a4f9aaa6b499b97c7834c0fbc8302

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Akademický článek

Direct binding of TFEα opens DNA binding cleft of RNA polymerase

Autor: Sung-Hoon Jun, Jaekyung Hyun, Jeong Seok Cha, Hoyoung Kim, Michael S. Bartlett, Hyun-Soo Cho, Katsuhiko S. Murakami

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)

How clamp conformation is regulated in the transcription cycle of stalk-containing archaeal and eukaryotic RNA polymerase (RNAP) systems is still not well understood. Here, the authors combine cryo-EM, X-ray crystallography and photo-crosslinking ass

Externí odkaz: https://doaj.org/article/9ace4ca1aed44741b400bd87ec0e18c7

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