Metabolomics-Driven Identification of the Rate-Limiting Steps in 1-Propanol Production

Autor: Toshiyuki Ohtake, Naoki Kawase, Sammy Pontrelli, Katsuaki Nitta, Walter A. Laviña, Claire R. Shen, Sastia P. Putri, James C. Liao, Eiichiro Fukusaki

Publikováno v: Frontiers in Microbiology, Vol 13 (2022)

The concerted effort for bioproduction of higher alcohols and other commodity chemicals has yielded a consortium of metabolic engineering techniques to identify targets to enhance performance of engineered microbial strains. Here, we demonstrate the

Externí odkaz: https://doaj.org/article/c6a22673e7084cbd859a2bdcd4b7bd60

Investigation of the effects of actinorhodin biosynthetic gene cluster expression and a rpoB point mutation on the metabolome of Streptomyces coelicolor M1146

Autor: Eriko Takano, Rainer Breitling, Katsuaki Nitta, Sastia Prama Putri, Eiichiro Fukusaki

Publikováno v: Journal of Bioscience and Bioengineering. 131:525-536

The previously reported Streptomyces coelicolor M1146 is commonly used as a host strain for engineering of secondary metabolite production. In this study, absolute quantification of intracellular and extracellular metabolites of M1146 was performed i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8e88fcfee741eb50182a11f02a93af9
https://doi.org/10.1016/j.jbiosc.2021.01.002

Multi-Omics Analysis of the Effect of cAMP on Actinorhodin Production in Streptomyces coelicolor

Autor: Eriko Takano, Sastia Prama Putri, Rainer Breitling, Eiichiro Fukusaki, Francesco Del Carratore, Katsuaki Nitta

Publikováno v: Nitta, K, Carratore, F D, Breitling, R, Takano, E, Putri, S P & Fukusaki, E 2020, ' Multi-Omics Analysis of the Effect of cAMP on Actinorhodin Production in Streptomyces coelicolor ', Frontiers in Bioengineering and Biotechnology, vol. 8 . https://doi.org/10.3389/fbioe.2020.595552
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
Frontiers in Bioengineering and Biotechnology

Cyclic adenosine monophosphate (cAMP) has been known to play an important role in regulating morphological development and antibiotic production in Streptomyces coelicolor. However, the functional connection between cAMP levels and antibiotic product

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::925e2fd49d4ec0535910e5c923e58d31
https://doi.org/10.3389/fbioe.2020.595552

Identifying metabolic elements that contribute to productivity of 1-propanol bioproduction using metabolomic analysis

Autor: Katsuaki Nitta, Eiichiro Fukusaki, Sammy Pontrelli, Sastia Prama Putri, James C. Liao, Shingo Noguchi, Takeshi Bamba, Claire R. Shen, Yasumune Nakayama

Publikováno v: Metabolomics : Official journal of the Metabolomic Society. 14(7)

Previously constructed Escherichia coli strains that produce 1-propanol use the native threonine pathway, or a heterologous citramalate pathway. However, based on the energy and cofactor requirements of each pathway, a combination of the two pathways

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d777a2e868b41c69e984935ca93142ac
https://pubmed.ncbi.nlm.nih.gov/30830363