Mammalian cell-based production of glycans, glycopeptides and glycomodules

Autor: Thapakorn Jaroentomeechai, Richard Karlsson, Felix Goerdeler, Fallen Kai Yik Teoh, Magnus Nørregaard Grønset, Dylan de Wit, Yen-Hsi Chen, Sanae Furukawa, Venetia Psomiadou, Ramon Hurtado-Guerrero, Elena Ethel Vidal-Calvo, Ali Salanti, Thomas J. Boltje, Leendert J. van den Bos, Christian Wunder, Ludger Johannes, Katrine T. Schjoldager, Hiren J. Joshi, Rebecca L. Miller, Henrik Clausen, Sergey Y. Vakhrushev, Yoshiki Narimatsu

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-16 (2024)

Abstract Access to defined glycans and glycoconjugates is pivotal for discovery, dissection, and harnessing of a range of biological functions orchestrated by cellular glycosylation processes and the glycome. We previously employed genetic glycoengin

Externí odkaz: https://doaj.org/article/c2a69eb2e97e4773a3813ca752d239d3

Fish-hunting cone snail disrupts prey’s glucose homeostasis with weaponized mimetics of somatostatin and insulin

Autor: Ho Yan Yeung, Iris Bea L. Ramiro, Daniel B. Andersen, Thomas Lund Koch, Alexander Hamilton, Walden E. Bjørn-Yoshimoto, Samuel Espino, Sergey Y. Vakhrushev, Kasper B. Pedersen, Noortje de Haan, Agnes L. Hipgrave Ederveen, Baldomero M. Olivera, Jakob G. Knudsen, Hans Bräuner-Osborne, Katrine T. Schjoldager, Jens Juul Holst, Helena Safavi-Hemami

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-16 (2024)

Abstract Venomous animals have evolved diverse molecular mechanisms to incapacitate prey and defend against predators. Most venom components disrupt nervous, locomotor, and cardiovascular systems or cause tissue damage. The discovery that certain fis

Externí odkaz: https://doaj.org/article/f2fa1c3f0aa445f0bdccf7fe579b75c1

An atlas of O-linked glycosylation on peptide hormones reveals diverse biological roles

Autor: Thomas D. Madsen, Lasse H. Hansen, John Hintze, Zilu Ye, Shifa Jebari, Daniel B. Andersen, Hiren J. Joshi, Tongzhong Ju, Jens P. Goetze, Cesar Martin, Mette M. Rosenkilde, Jens J. Holst, Rune E. Kuhre, Christoffer K. Goth, Sergey Y. Vakhrushev, Katrine T. Schjoldager

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)

O-glycosylation is an abundant post-translational modification but its relevance for bioactive peptides is unclear. Here, the authors detect O-glycans on almost one third of the classified peptide hormones and show that O-glycosylation can modulate p

Externí odkaz: https://doaj.org/article/d81560792a8349198626ee05e6f9d81f

The SHDRA syndrome-associated gene TMEM260 encodes a protein-specific O-mannosyltransferase

Autor: Ida Signe Bohse Larsen, Lorenzo Povolo, Luping Zhou, Weihua Tian, Kasper Johansen Mygind, John Hintze, Chen Jiang, Verity Hartill, Katrina Prescott, Colin A. Johnson, Sureni V. Mullegama, Allyn McConkie-Rosell, Marie McDonald, Lars Hansen, Sergey Y. Vakhrushev, Katrine T. Schjoldager, Henrik Clausen, Thomas Worzfeld, Hiren J. Joshi, Adnan Halim

Publikováno v: Larsen, I S B, Povolo, L, Zhou, L, Tian, W, Mygind, K J, Hintze, J, Jiang, C, Hartill, V, Prescott, K, Johnson, C A, Mullegama, S V, McConkie-Rosell, A, McDonald, M, Hansen, L, Vakhrushev, S Y, Schjoldager, K T, Clausen, H, Worzfeld, T, Joshi, H J & Halim, A 2023, ' The SHDRA syndrome-associated gene TMEM260 encodes a protein-specific O-mannosyltransferase ', Proceedings of the National Academy of Sciences of the United States of America, vol. 120, no. 21, pp. e2302584120 . https://doi.org/10.1073/pnas.2302584120

Mutations in the TMEM260 gene cause structural heart defects and renal anomalies syndrome, but the function of the encoded protein remains unknown. We previously reported wide occurrence of O-mannose glycans on extracellular immunoglobulin, plexin, t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::002d22943d971c680b2f7b4e379c7c4f
https://doi.org/10.1073/pnas.2302584120

A Bump-and-Hole Approach to Dissect Regulation of Protein O-Glycosylation

Autor: Henrik Clausen, Ramon Hurtado-Guerrero, Katrine T. Schjoldager

Publikováno v: Molecular Cell. 78:803-805

In this issue of Molecular Cell, Schumann et al. (2020) present a novel strategy to dissect the regulation of protein O-glycosylation by a large family of isoenzymes in cells. They employ a bump-and-hole engineering approach to capture the specific c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f2daf786868040e861699e0d368657df
https://doi.org/10.1016/j.molcel.2020.05.019