In through the Out Door: A Functional Virulence Factor Secretion System Is Necessary for Phage Infection in Ralstonia solanacearum

Autor: André da Silva Xavier, Alessandra G. de Melo, Connor G. Hendrich, Denise M. Tremblay, Geneviève M. Rousseau, Pier-Luc Plante, Katrina T. Forest, Poliane Alfenas-Zerbini, Caitilyn Allen, Sylvain Moineau

Publikováno v: mBio, Vol 13, Iss 6 (2022)

ABSTRACT Bacteriophages put intense selective pressure on microbes, which must evolve diverse resistance mechanisms to survive continuous phage attacks. We used a library of spontaneous Bacteriophage Insensitive Mutants (BIMs) to learn how the plant

Externí odkaz: https://doaj.org/article/23858f925a7146aa8fd267b16105d7b4

A Widespread Bacterial Secretion System with Diverse Substrates

Autor: Alex S. Grossman, Terra J. Mauer, Katrina T. Forest, Heidi Goodrich-Blair

Publikováno v: mBio, Vol 12, Iss 4 (2021)

ABSTRACT In host-associated bacteria, surface and secreted proteins mediate acquisition of nutrients, interactions with host cells, and specificity of tissue localization. In Gram-negative bacteria, the mechanism by which many proteins cross and/or b

Externí odkaz: https://doaj.org/article/361bdb1d08f840cf9fc9bf73e05bb91e

Unraveling the Self-Assembly of the Pseudomonas aeruginosa XcpQ Secretin Periplasmic Domain Provides New Molecular Insights into Type II Secretion System Secreton Architecture and Dynamics

Autor: Badreddine Douzi, Nhung T. T. Trinh, Sandra Michel-Souzy, Aline Desmyter, Geneviève Ball, Pascale Barbier, Artemis Kosta, Eric Durand, Katrina T. Forest, Christian Cambillau, Alain Roussel, Romé Voulhoux

Publikováno v: mBio, Vol 8, Iss 5 (2017)

ABSTRACT The type II secretion system (T2SS) releases large folded exoproteins across the envelope of many Gram-negative pathogens. This secretion process therefore requires specific gating, interacting, and dynamics properties mainly operated by a b

Externí odkaz: https://doaj.org/article/bf8f42205505467f8798d46d021e96ac

CbpD crystal structure adds intrigue to substrate-specificity motifs in chitin-active lytic polysaccharide monooxygenases

Autor: Christopher M. Dade, Badreddine Douzi, Cristian Cambillau, Genevieve Ball, Romé Voulhoux, Katrina T. Forest

Pseudomonas aeruginosa secretes diverse proteins via its Type 2 Secretion System, including a 39 KDa Chitin-Binding Protein, CbpD. CbpD was recently shown to be a lytic polysaccharide monooxygenase active on chitin, and to contribute substantially to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::029e25a88f832024bdc3ffff54e43503
https://doi.org/10.1101/2022.04.15.488525

The crystal structure of CbpD clarifies substrate-specificity motifs in chitin-active lytic polysaccharide monooxygenases

Autor: Christopher M. Dade, Badreddine Douzi, Christian Cambillau, Genevieve Ball, Romé Voulhoux, Katrina T. Forest

Publikováno v: Acta crystallographica Section D : Structural biology [1993-...]
Acta crystallographica Section D : Structural biology [1993-..], 2022, 78 (8), pp.1064-1078. ⟨10.1107/S2059798322007033⟩

Pseudomonas aeruginosa secretes diverse proteins via its type 2 secretion system, including a 39 kDa chitin-binding protein, CbpD. CbpD has recently been shown to be a lytic polysaccharide monooxygenase active on chitin and to contribute substantiall

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb869f94899dc1aff80c91f327ef4407
https://hal.science/hal-03739502/file/jc5051.pdf