An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein

Autor: Pooran Singh Dewari, Benjamin Southgate, Katrina Mccarten, German Monogarov, Eoghan O'Duibhir, Niall Quinn, Ashley Tyrer, Marie-Christin Leitner, Colin Plumb, Maria Kalantzaki, Carla Blin, Rebecca Finch, Raul Bardini Bressan, Gillian Morrison, Ashley M Jacobi, Mark A Behlke, Alex von Kriegsheim, Simon Tomlinson, Jeroen Krijgsveld, Steven M Pollard

Publikováno v: eLife, Vol 7 (2018)

CRISPR/Cas9 can be used for precise genetic knock-in of epitope tags into endogenous genes, simplifying experimental analysis of protein function. However, Cas9-assisted epitope tagging in primary mammalian cell cultures is often inefficient and reli

Externí odkaz: https://doaj.org/article/75965eb632694dcb913174d34eba59b9

An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein

Autor: German Monogarov, Eoghan O'Duibhir, Alex von Kriegsheim, Jeroen Krijgsveld, Marie-Christin Leitner, Carla Blin, Niall Quinn, Simon R. Tomlinson, Pooran Singh Dewari, Mark A. Behlke, Gillian Morrison, Maria Kalantzaki, Ashley M. Jacobi, Ashley Tyrer, Steven M. Pollard, Rebecca Finch, Katrina Mccarten, Raul Bardini Bressan, Benjamin Southgate, Colin Plumb

Publikováno v: Dewari, P S, Southgate, B, Mccarten, K, Monogarov, G, O'Duibhir, E, Quinn, N, Tyrer, A, Leitner, M-C, Plumb, C, Kalantzaki, M, Blin, C, Finch, R, Bressan, R B, Morrison, G, Jacobi, A M, Behlke, M A, von Kriegsheim, A, Tomlinson, S, Krijgsveld, J & Pollard, S M 2018, ' An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein ', eLIFE, vol. 7 . https://doi.org/10.7554/eLife.35069
Dewari, P, Southgate, B, McCarten, K, Monogarov, G, O'Duibhir, E, Quinn, N, Tyrer, A, Leitner, M-C, Plumb, C, Kalantzaki, M, Blin, C, Finch, R, Bressan, R, Morrison, G, Jacobi, A, Behlke, M, von Kriegsheim, A, Tomlinson, S, Krijgsveld, J & Pollard, S 2018, ' An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein ', eLife, vol. 7, no. e35069 . https://doi.org/10.7554/eLife.35069
eLife
eLife, Vol 7 (2018)

CRISPR/Cas9 can be used for precise genetic knock-in of epitope tags into endogenous genes, simplifying experimental analysis of protein function. However, Cas9-assisted epitope tagging in primary mammalian cell cultures is often inefficient and reli

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e795d5d76b4fae4973d1634b361cb13
https://www.pure.ed.ac.uk/ws/files/59253944/elife_35069_v1_1_.pdf