Zobrazeno 1 - 10
of 63
pro vyhledávání: '"Katrin Messerschmidt"'
Publikováno v:
Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-12 (2020)
Abstract Background Enforced ATP wasting has been recognized as a promising metabolic engineering strategy to enhance the microbial production of metabolites that are coupled to ATP generation. It also appears to be a suitable approach to improve pro
Externí odkaz:
https://doaj.org/article/64f5503a85bb45e7947444d0d5d58dee
Autor:
Christoph Rieck, Daniel Geiger, Jennifer Munkert, Katrin Messerschmidt, Jan Petersen, Juliane Strasser, Nadine Meitinger, Wolfgang Kreis
Publikováno v:
MicrobiologyOpen, Vol 8, Iss 12, Pp n/a-n/a (2019)
Abstract A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ5
Externí odkaz:
https://doaj.org/article/66f285ad80934f78860eb73c4d014a34
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
The International Synthetic Yeast Sc2.0 project has built Cre recombinase sites into synthetic chromosomes, enabling rapid genome evolution. Here the authors demonstrate L-SCRaMbLE, a light-controlled recombinase tool with improved control over recom
Externí odkaz:
https://doaj.org/article/d26c1b18aa1343b1b6443c240f39a31a
Publikováno v:
Applied Sciences, Vol 11, Iss 20, p 9359 (2021)
Monoclonal antibodies are used worldwide as highly potent and efficient detection reagents for research and diagnostic applications. Nevertheless, the specific targeting of complex antigens such as whole microorganisms remains a challenge. To provide
Externí odkaz:
https://doaj.org/article/0a4b8532bf70426da2c3d6c2c7ba55f6
Publikováno v:
PLoS ONE, Vol 13, Iss 1, p e0190526 (2018)
Cloning multiple DNA fragments for delivery of several genes of interest into the plant genome is one of the main technological challenges in plant synthetic biology. Despite several modular assembly methods developed in recent years, the plant biote
Externí odkaz:
https://doaj.org/article/6fb3b9798171451fba472eb2bd9eca73
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 5 (2017)
Orthogonal systems for heterologous protein expression as well as for the engineering of synthetic gene regulatory circuits in hosts like Saccharomyces cerevisiae depend on synthetic transcription factors (synTFs) and corresponding cis-regulatory bin
Externí odkaz:
https://doaj.org/article/27db4c8594de4437ba43909699f5699e
Publikováno v:
Biotechnology for Biofuels
Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-12 (2020)
Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-12 (2020)
Background Enforced ATP wasting has been recognized as a promising metabolic engineering strategy to enhance the microbial production of metabolites that are coupled to ATP generation. It also appears to be a suitable approach to improve production o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::493f6b2b70cf2f6f23e308c5a669ff61
https://hdl.handle.net/21.11116/0000-0007-6D82-C21.11116/0000-0007-6D84-A
https://hdl.handle.net/21.11116/0000-0007-6D82-C21.11116/0000-0007-6D84-A
Publikováno v:
ACS Synthetic Biology
[Image: see text] One-carbon (C1) compounds are attractive microbial feedstocks as they can be efficiently produced from widely available resources. Formate, in particular, represents a promising growth substrate, as it can be generated from electroc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c1b8e1a9750e0f9b22bc01f774dfe21
https://europepmc.org/articles/PMC6528164/
https://europepmc.org/articles/PMC6528164/
Publikováno v:
Analytical Biochemistry. 509:24-32
Synthetic biology aims at designing and engineering organisms. The engineering process typically requires the establishment of suitable DNA constructs generated through fusion of multiple protein coding and regulatory sequences. Conventional cloning
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70a45280ff822fb20422e6fe373430c6
https://doi.org/10.1016/j.ab.2016.05.029
https://doi.org/10.1016/j.ab.2016.05.029
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
The synthetic yeast genome constructed by the International Synthetic Yeast Sc2.0 consortium adds thousands of loxPsym recombination sites to all 16 redesigned chromosomes, allowing the shuffling of Sc2.0 chromosome parts by the Cre-loxP recombinatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24d5519ace981e3436533deea9fb3f66
https://doi.org/10.1038/s41467-017-02208-6
https://doi.org/10.1038/s41467-017-02208-6