A CRISPR/Cas9-Based Mutagenesis Protocol for Brachypodium distachyon and Its Allopolyploid Relative, Brachypodium hybridum

Autor: Karolina Hus, Alexander Betekhtin, Artur Pinski, Magdalena Rojek-Jelonek, Ewa Grzebelus, Candida Nibau, Mingjun Gao, Katja E. Jaeger, Glyn Jenkins, John H. Doonan, Robert Hasterok

Publikováno v: Frontiers in Plant Science, Vol 11 (2020)

The CRISPR/Cas9 system enables precise genome editing and is a useful tool for functional genomic studies. Here we report a detailed protocol for targeted genome editing in the model grass Brachypodium distachyon and its allotetraploid relative B. hy

Externí odkaz: https://doaj.org/article/d0cb9eb991fb4d399fbf99e7362a8cd1

The evening complex integrates photoperiod signals to control flowering in rice

Autor: Luis Andrade, Yunlong Lu, André Cordeiro, João M. F. Costa, Philip A. Wigge, Nelson J. M. Saibo, Katja E. Jaeger

Publikováno v: Proceedings of the National Academy of Sciences. 119

Plants use photoperiodism to activate flowering in response to a particular daylength. In rice, flowering is accelerated in short-day conditions, and even a brief exposure to light during the dark period (night-break) is sufficient to delay flowering

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5a8472b641ff2ac083c8e7e87d2bc14
https://doi.org/10.1073/pnas.2122582119

Warm Temperature Promotes Shoot Regeneration in Arabidopsis thaliana

Autor: Alice Lambolez, Ayako Kawamura, Tatsuya Takahashi, Bart Rymen, Akira Iwase, David S Favero, Momoko Ikeuchi, Takamasa Suzuki, Sandra Cortijo, Katja E Jaeger, Philip A Wigge, Keiko Sugimoto

Publikováno v: Plantcell physiology. 63(5)

Many plants are able to regenerate upon cutting, and this process can be enhanced in vitro by incubating explants on hormone-supplemented media. While such protocols have been used for decades, little is known about the molecular details of how incub

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9849f1ca1b6b523aa66c58b5442e482
https://pubmed.ncbi.nlm.nih.gov/35157760

An early-morning gene network controlled by phytochromes and cryptochromes regulates photomorphogenesis pathways in Arabidopsis

Autor: Martin Balcerowicz, Philip A. Wigge, Daphne Ezer, Hui Lan, Susana Conde, Katja E. Jaeger, Dorothee Stoeckle, Mahiar Mahjoub, Duy Nguyen

Publikováno v: Molecular plant. 14(6)

Light perception at dawn plays a key role in coordinating multiple molecular processes and in entraining the plant circadian clock. The Arabidopsis mutant lacking the main photoreceptors, however, still shows clock entrainment, indicating that the in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffbbf2744a11d69b4c187c61dba002a4
https://pubmed.ncbi.nlm.nih.gov/33766657

A prion-like domain in ELF3 functions as a thermosensor in Arabidopsis

Autor: Philip A. Wigge, Antonio Daniel Barbosa, Mingjun Gao, Stephanie Hutin, Elodie Pierre, Feng Geng, Xuelei Lai, Dorothee Derwort, Catarina S. Silva, Sol-Bi Kim, Katja E. Jaeger, Sujeong Baek, Janet R. Kumita, Chloe Zubieta, Jaehoon Jung

Publikováno v: Nature
Nature, 2020, 585 (7824), pp.256-260. ⟨10.1038/s41586-020-2644-7⟩
Nature, Nature Publishing Group, 2020, 585 (7824), pp.256-260. ⟨10.1038/s41586-020-2644-7⟩

International audience; Temperature controls plant growth and development, and climate change has already altered the phenology of wild plants and crops1. However, the mechanisms by which plants sense temperature are not well understood. The evening

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eaf1a5e0af1d1cad76791514db668986
https://hal.science/hal-02954052/file/Jung_et_al_2020_draft.pdf