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Autor:
William C. Skarnes, Sajith Perera, Roxana C. Mustata, Katie Tremble, José C. R. Silva, Tiago Grego, Katie Andrews, Jihoon Kim, Juergen Fink, Alessandra Merenda, Bon-Kyoung Koo, Amanda Andersson-Rolf
Publikováno v:
Nature methods
Loss-of-function studies are key for investigating gene function, and CRISPR technology has made genome editing widely accessible in model organisms and cells. However, conditional gene inactivation in diploid cells is still difficult to achieve. Her