Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Katie A. Carnes"'
Autor:
Roberto F. Delgadillo, Katie A. Carnes, Nestor Valles-Villarreal, Omar Olmos, Kathia Zaleta-Rivera, Lawrence J. Parkhurst
Publikováno v:
Biosensors, Vol 10, Iss 11, p 180 (2020)
The Stopped-Flow apparatus (SF) tracks molecular events by mixing the reactants in sub-millisecond regimes. The reaction of intrinsically or extrinsically labeled biomolecules can be monitored by recording the fluorescence, F(t), anisotropy, r(t), po
Externí odkaz:
https://doaj.org/article/7dfaeab36ec547de87ec5e260492acf3
Autor:
Roberto F Delgadillo, Timothy C Mueser, Kathia Zaleta-Rivera, Katie A Carnes, José González-Valdez, Lawrence J Parkhurst
Publikováno v:
PLoS ONE, Vol 14, Iss 2, p e0204194 (2019)
The high affinity (KD ~ 10-15 M) of biotin for avidin and streptavidin is the essential component in a multitude of bioassays with many experiments using biotin modifications to invoke coupling. Equilibration times suggested for these assays assume t
Externí odkaz:
https://doaj.org/article/fb077640e6a6488c9760c8c1768cf7ce
Autor:
Katie A. Carnes, Lee D. Oliver, Timothy A. Brown, Roberto F. Delgadillo, Michelle S. Ward, Sina Mortazavi, Michael J. Morris, Justin W. Shearer, Joshua S. Fuller
Polysorbate (also known as “Tween”) 80 (PS80) is the most common surfactant in biopharmaceuticals. Here, we report a sensitive, and specific platform analytical method using reversed-phase high performance liquid chromatography employing a charge
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e43ebec61433c41a7c8d8378a5ebe04
Autor:
Katie A. Carnes, Lawrence J. Parkhurst, Kathia Zaleta-Rivera, Omar Olmos, Roberto F. Delgadillo
Publikováno v:
Analytical Chemistry
Time-resolved donor-detected Forster resonance energy transfer (trDDFRET) allows the observation of molecular interactions of dye-labeled biomolecules in the ∼10-100 A region. However, we can observe longer-range interactions when using time-resolv
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb2efca23cbd03d418a3d1763c99a910
http://europepmc.org/articles/PMC7992049
http://europepmc.org/articles/PMC7992049
Autor:
Timothy C. Mueser, Katie A. Carnes, Lawrence J. Parkhurst, Kathia Zaleta-Rivera, José González-Valdez, Roberto F. Delgadillo
Publikováno v:
PLoS ONE, Vol 14, Iss 2, p e0204194 (2019)
PLoS ONE
PLoS ONE
The high affinity (KD ~ 10−15 M) of biotin for avidin and streptavidin is the essential component in a multitude of bioassays with many experiments using biotin modifications to invoke coupling. Equilibration times suggested for these assays assume
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73039636a968c86dff7b47ff33d34af0
https://doaj.org/article/fb077640e6a6488c9760c8c1768cf7ce
https://doaj.org/article/fb077640e6a6488c9760c8c1768cf7ce
Autor:
Katie A. Carnes, Roberto F. Delgadillo, Kathia Zaleta-Rivera, José González-Valdez, Lawrence J. Parkhurst, Timothy C. Mueser
The high affinity (KD∼ 10−15M) of biotin to avidin and streptavidin is the essential component in a multitude of bioassays with many experiments using biotin modifications to invoke coupling. Equilibration times suggested for these assays assume
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85d2c53cabe9fa1a3e9127d0570f7de6
https://doi.org/10.1101/410548
https://doi.org/10.1101/410548
Autor:
Jason G. Glanzer, Shengqin Liu, Lawrence J. Parkhurst, Gregory G. Oakley, Patricia Soto, Katie A. Carnes
Publikováno v:
Nucleic Acids Research
Replication protein A (RPA), essential for DNA replication, repair and DNA damage signalling, possesses six ssDNA-binding domains (DBDs), including DBD-F on the N-terminus of the largest subunit, RPA70. This domain functions as a binding site for p53
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2759bd3a67bcd0426ae19401be622eb6
http://europepmc.org/articles/PMC3561959
http://europepmc.org/articles/PMC3561959
Publikováno v:
Biophysical Journal. 102(3)
The on-rate constants (kon) of biotin (B) binding to avidin (AV) and streptavidin (SAV) are believed to be diffusion limited (109 M−1s−1). In this study; we asked whether these reactions were actual diffusion controlled, what association model an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6937d3fd48ab54a90a97cad0f6638579