A Quantitative Genetic Interaction Map of HIV Infection

Autor: David C. Crosby, Assen Roguev, Joshua Kane, Michael Shales, Jiewei Xu, Erica Stevenson, Ariane Watson, Alexander Marson, David E. Gordon, Ivan Marazzi, Nevan J. Krogan, Gwendolyn M. Jang, Simin Zheng, Jeffrey Z. Guo, Danielle L. Swaney, Alan D. Frankel, Erik Verschueren, Gerard Cagney, Kathy Franks-Skiba

Publikováno v: Molecular cell, vol 78, iss 2
Mol Cell

We have developed a platform for quantitative genetic interaction mapping using viral infectivity as a functional readout and constructed a viral host-dependency epistasis map (vE-MAP) of 356 human genes linked to HIV function, comprising >63,000 pai

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8289265b3b0e39fd524b7ea07648e0d0
https://escholarship.org/uc/item/75v3v7mq

Depletion of Phosphate in Active Muscle Fibers Probes Actomyosin States within the Powerstroke

Autor: Roger Cooke, Kathy Franks-Skiba, E. Pate

Publikováno v: Biophysical Journal. 74(1):369-380

Variation in the concentration of orthophosphate (Pi) in actively contracting, chemically skinned muscle fibers has proved to be a useful probe of actomyosin interaction. Previous studies have shown that isometric tension (Po) decreases linearly in t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da5a7ec70e7c32683e8a0fda508a3521

Reversible inactivation of myosin subfragment 1 activity by mechanical immobilization

Autor: Kelly Duignan, Stefan Highsmith, Kathy Franks-Skiba, Katherine Polosukhina, Roger Cooke

The Mg-ATPase activity of skeletal muscle myosin subfragment 1 (S1) is reversibly eliminated when it is aggregated by the force of osmotic pressure dehydration using polyethylene glycol (PEG). Several experiments indicate nucleotides bind aggregated

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d87b7bb553ccd161292e0a69d7424543
https://europepmc.org/articles/PMC1299492/

Myosin regulatory light chain phosphorylation inhibits shortening velocities of skeletal muscle fibers in the presence of the myosin inhibitor blebbistatin

Autor: Roger Cooke, Kathy Franks-Skiba, Melanie A. Stewart

Publikováno v: Stewart, Melanie; Franks-Skiba, Kathy; & Cooke, Roger. (2009). Myosin regulatory light chain phosphorylation inhibits shortening velocities of skeletal muscle fibers in the presence of the myosin inhibitor blebbistatin. Journal of Muscle Research and Cell Motility, 30(1), pp 17-27. doi: 10.1007/s10974-008-9162-9. Retrieved from: http://www.escholarship.org/uc/item/075661nn

Phosphorylation of skeletal myosin regulatory light chain (RLC) occurs in fatigue and may play a role in the inhibition of shortening velocities observed in vivo. Forces and shortening velocities were measured in permeabilized rabbit psoas fibers wit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd2c9e2344bb7188d3a2dabf504b267d