Spindle Position Checkpoint Kinase Kin4 Regulates Organelle Transport in Saccharomyces cerevisiae

Autor: Lakhan Ekal, Abdulaziz M. S. Alqahtani, Maya Schuldiner, Einat Zalckvar, Ewald H. Hettema, Kathryn R. Ayscough

Publikováno v: Biomolecules, Vol 13, Iss 7, p 1098 (2023)

Membrane-bound organelles play important, frequently essential, roles in cellular metabolism in eukaryotes. Hence, cells have evolved molecular mechanisms to closely monitor organelle dynamics and maintenance. The actin cytoskeleton plays a vital rol

Externí odkaz: https://doaj.org/article/a63eb1d2dfa1405eacf015e417b8f0d2

Mutation of key lysine residues in the Insert B region of the yeast dynamin Vps1 disrupts lipid binding and causes defects in endocytosis.

Autor: Iwona I Smaczynska-de Rooij, Christopher J Marklew, Sarah E Palmer, Ellen G Allwood, Kathryn R Ayscough

Publikováno v: PLoS ONE, Vol 14, Iss 4, p e0215102 (2019)

The yeast dynamin-like protein Vps1 has roles at multiple stages of membrane trafficking including Golgi to vacuole transport, endosomal recycling, endocytosis and in peroxisomal fission. While the majority of the Vps1 amino acid sequence shows a hig

Externí odkaz: https://doaj.org/article/a72d29a976a94208aab5e5914b6ec298

Tracking autophagy during proliferation and differentiation of Trypanosoma brucei

Autor: Laila Moustaq, Iwona I. Smaczynska-de Rooij, Sarah E. Palmer, Christopher J. Marklew, Kathryn R. Ayscough

Publikováno v: Microbial Cell, Vol 3, Iss 4, Pp 147-158 (2016)

The dynamins represent a superfamily of proteins that have been shown to function in a wide range of membrane fusion and fission events. An increasing number of mutations in the human classical dynamins, Dyn-1 and Dyn-2 has been reported, with diseas

Externí odkaz: https://doaj.org/article/f8c0e1bb87b046d48a51e00b11427e5a

The dynamin Vps1 mediates Atg9 transport to the sites of autophagosome formation

Autor: Henning Arlt, Babu Raman, Yasmina Filali-Mouncef, Yan Hu, Alexandre Leytens, Ralph Hardenberg, Rodrigo Guimarães, Franziska Kriegenburg, Muriel Mari, Iwona I. Smaczynska-de Rooij, Kathryn R. Ayscough, Jörn Dengjel, Christian Ungermann, Fulvio Reggiori

Publikováno v: Arlt, H, Raman, B, Filali-Mouncef, Y, Hu, Y, Leytens, A, Hardenberg, R, Guimarães, R, Kriegenburg, F, Mari, M, Smaczynska-de Rooij, I I, Ayscough, K R, Dengjel, J, Ungermann, C & Reggiori, F 2023, ' The dynamin Vps1 mediates Atg9 transport to the sites of autophagosome formation ', The Journal of Biological Chemistry, vol. 299, no. 5, 104712 . https://doi.org/10.1016/j.jbc.2023.104712

Autophagy is a key process in eukaryotes to maintain cellular homeostasis by delivering cellular components to lysosomes/vacuoles for degradation and reuse of the resulting metabolites. Membrane rearrangements and trafficking events are mediated by t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38d1962a9b94bc41951c033a9644fb97
https://doi.org/10.1016/j.jbc.2023.104712

Elucidating Key Motifs Required for Arp2/3-Dependent and Independent Actin Nucleation by Las17/WASP.

Autor: Ellen G Allwood, Joe J Tyler, Agnieszka N Urbanek, Iwona I Smaczynska-de Rooij, Kathryn R Ayscough

Publikováno v: PLoS ONE, Vol 11, Iss 9, p e0163177 (2016)

Actin nucleation is the key rate limiting step in the process of actin polymerization, and tight regulation of this process is critical to ensure actin filaments form only at specific times and at defined regions of the cell. Arp2/3 is a well-charact

Externí odkaz: https://doaj.org/article/695b3292900f42cb8a86695a33e7ea41