Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Kathleen Prinzo"'
Autor:
Edgar P. Heimer, J. E. Scheffler, Anthony Neri, Joseph E. LoSardo, Eva Bekesi, Kathleen Prinzo
Publikováno v:
International Journal of Peptide and Protein Research. 45:194-199
Guanosine triphosphatase activating protein (GAP) is an important modulator of p21ras (Ras)-dependent signal transduction in mammalian cells and in insulin-induced maturation of Xenopus oocytes. A synthetic octapeptide from the catalytic domain of GA
Autor:
Kathleen Prinzo, Joseph E. LoSardo, B. Wegrzynski, S.E. Kiefer, S. D. Emerson, J. E. Scheffler, Kwei-Lan Tsao, Anthony Neri, Eva Bekesi, David S. Waugh
Publikováno v:
Journal of Biological Chemistry. 269:22340-22346
Four overlapping peptide fragments of human c-Raf-1 (residues 55-132, 55-117, 77-132, and 77-117) were expressed in Escherichia coli as carboxyl-terminal extensions of maltose binding protein (MBP). The MBP-Raf fusions were purified by affinity chrom
Scintillation proximity assay to measure the binding of ras-GTP to the ras-binding domain of c-Raf-1
Publisher Summary This chapter describes the scintillation proximity assay (SPA) to measure the binding of Ras-GTP to the Ras-binding domain of c-Raf-1. By capturing the protein-bound [ 3 H]GTP on SPA beads, it is possible to measure the binding of R
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4df209203cbedfa227732c26a525325c
https://doi.org/10.1016/s1080-8914(96)80014-7
https://doi.org/10.1016/s1080-8914(96)80014-7
Publikováno v:
Biochemistry. 33(25)
Raf-1 is a 74-kDa serine-threonine kinase which serves as the immediate downstream target of Ras in the cell growth signal transduction pathway. Recent genetic and biochemical experiments have demonstrated that (1) Ras interacts directly with the ami