Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Kathleen L. Tivel"'
Autor:
Martin K. Bijsterbosch, Erik T. Rump, Muthiah Manoharan, Kathleen L. Tivel, Richard van Veghel, Rick Dorland, Erik A.L. Biessen, Theo J.C. Van Berkel, Remco L. A. de Vrueh
Publikováno v:
Nucleic Acids Research. 28:2717-2725
Several studies have shown improved efficacy of cholesteryl-conjugated phosphorothioate antisense oligodeoxynucleotides. To gain insight into the mechanisms of the improved efficacy in vivo, we investigated the disposition of ISIS-9388, the 3'-choles
Autor:
P. Dan Cook, Martin K. Bijsterbosch, Muthiah Manoharan, Erik T. Rump, Kathleen L. Tivel, Remco L. A. de Vrueh, Theo J.C. Van Berkel, Erik A.L. Biessen
Publikováno v:
Nucleosides and Nucleotides. 16:1165-1168
A cholesterol-conjugated phosphorothioate ICAM-1 antisense oligo-nucleotide was evaluated for its binding to lipoproteins and its biodistribution. Our study indicates that the conjugate behaves differently from the parent compound.
Autor:
Isabelle Barber-Peoc'h, Mark J. Graham, Muthiah Manoharan, Gopal B. Inamati, P. Dan Cook, Shefali Shah, Stanley T. Crooke, Kathleen L. Tivel, Laura K. Andrade, C. Frank Bennett, Thomas P. Condon, Venkatraman Mohan
Publikováno v:
Nucleosides and Nucleotides. 16:1129-1138
We have employed chemical modification strategies to improve cellular ahsorption of oligonucleotides. These include the conjugation of various pendant moieties to the oligonucleotide to affect its overall physical properties such as hydrophobicity, c
Publikováno v:
Tetrahedron Letters. 36:3651-3654
Lipophilic nucleosides were synthesized from uridine via either a 2′- O -hexylamino tether or the corresponding 3′-linker. These synthons allowed site-specific incorporation of the lipophilic moieties into antisense oligonucleotides. A spectrum o
Publikováno v:
Tetrahedron Letters. 36:3647-3650
Nucleoside synthons carrying an aminohexyl moiety tethered at the 2′- O - or 3′- O - positions of uridine have been synthesized and incorporated into oligonucleotides. The aminohexyl tether was used to conjugate functionalities such as intercalat
Autor:
Laura K. Andrade, P. Dan Cook, C. Frank Bennett, Thomas P. Condon, Venkatraman Mohan, Muthiah Manoharan, Kathleen L. Tivel
Publikováno v:
Nucleosides, Nucleotides and Nucleic Acids. 14:969-973
Cholic acid, cholesterol, several polyamines and polyethylene glycols were conjugated to antisense oligonucleotides targeted to human or murine intercellular adhesion molecule-1 (ICAM-1) mRNA to study their effects on cellular absorption.
Publikováno v:
Gene. 149:147-156
A 2prime;-O-hexylthiotrityl adenosine phosphoramidite has been synthesized and incorporated into oligodeoxyribonucleotide (oligo) phosphodiesters and phosphorothioates. These oligos possess the lipophilic 2prime;-O-hexylthiotrityl group at pre-select
Publikováno v:
Bioorganic & Medicinal Chemistry Letters. 3:2765-2770
A 2′- O -hexylthiotrityl adenosine phosphoramidite has been synthesized and incorporated into oligonucleotide phosphodiesters and phosphorothioates. This tether has potential antisense applications, offers a convenient nucleophile for conjugation o
Autor:
Gopal B. Inamati, Bruce S. Ross, Muthiah Manoharan, Kathleen L. Tivel, P. Dan Cook, Boyd Conklin
Publikováno v:
Nucleosides and Nucleotides. 16:1141-1143
Recently our laboratory introduced1 chemistries to synthesize 2′- and 3′- cholesteroluridine conjugates which were incorporated into several antisense oligonucleotides. We have now extended this chemistry to other nucleosides (adenosine and cytos
2′- and 3′- Biotin Conjugated Nucleoside Building Blocks: Synthesis of Biotinylated Oligonucleotides
Autor:
Patrick Wheeler, Muthiah Manoharan, P. Dan Cook, Gopal B. Inamati, Kim Stecker, Kathleen L. Tivel
Publikováno v:
Nucleosides and Nucleotides. 16:1411-1413
The vitamin biotin plays a significant role in biological assays based on its unusually high affinity [KD=10−15M] to streptavidin and avidin. This assay can be used for monitoring cellular trafficking of antisense oligonucleotides using hiotin conj