Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Kathleen A. Denis"'
Autor:
Louis P. Berneman, Kathleen A. Denis
Publikováno v:
Industry and Higher Education. 12:202-205
The dramatic rise of activity in academic–industry technology transfer in the USA, and its increasingly significant impact, lead the authors to examine changes, trends, and recent developments in transfer activities. They examine a variety of issue
Publikováno v:
Developmental Immunology
Mice transgenic forγ2b Ig heavy chain were examined for alterations in B-cell differentiation and endogenous Ig gene rearrangement and expression. Fresh bone marrow from these mice was markedly reduced in BP-1+cells and there were small reductions i
Publikováno v:
Journal of Allergy and Clinical Immunology. 87:262
Autor:
Norman R. Klinman, Kathleen A. Denis
Publikováno v:
The Journal of Experimental Medicine
Two hybridoma cell lines were established with B cells derived from neonatal BALB/c spleen cells. The anti-dinitrophenyl (DNP) antibodies derived from these lines were characterized with respect to their isotype, affinity, and isoelectric point. Anti
Autor:
David Lo, Peter Engler, Katherine A. Gollahon, Joanna Manz, Ralph L. Brinster, Kathleen A. Denis, Ursula Storb
Publikováno v:
Annals of the New York Academy of Sciences. 546:51-56
Immunoglobulin (Ig) genes are expressed sequentially (first H-, then L-chain genes) during the development of B lymphocytes. These studies, performed with transgenic mice and transfected cells, were aimed at the regulation of turning on and off the r
Autor:
Kathleen A. Denis, Owen N. Witte
Publikováno v:
Proceedings of the National Academy of Sciences. 83:441-445
An in vitro system for the study of B-lymphocyte differentiation from precursor elements has been established. Two separate sets of culture conditions are utilized to control B-lymphocyte precursor growth versus differentiation. The first, modeled af
Publikováno v:
Proceedings of the National Academy of Sciences. 81:1292-1296
We determined that mouse lymphoid cell lines can be transfected at high efficiencies (10-70%) by a polyoma virus shuttle vector. With this vector, we obtained expression of a cloned mouse alpha heavy chain gene transfected into cell lines representat
Publikováno v:
Nature. 316:356-358
Recent experiments have shown that the microinjected kappa-chain gene of transgenic mice is expressed in a tissue-specific fashion only in B lymphocytes. The next step was to determine whether, within the B-lymphocyte lineage, the kappa-chain gene wa
Publikováno v:
Annual review of immunology. 3
The development of B lymphocytes provides a useful model system for the examination of factors that regulate growth and differentiation. The wealth of information on the molecular biology of immunoglobulin gene structure and expression (1, 2) coupled