Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Katherine J. Robins"'
Autor:
Wei Li Thong, Yingxin Zhang, Ying Zhuo, Katherine J. Robins, Joanna K. Fyans, Abigail J. Herbert, Brian J. C. Law, Jason Micklefield
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
Engineering biosynthetic assembly lines is a powerful path to new natural products but is challenging with current methods. Here the authors use CRISPR-Cas9 to exchange subdomains within NRPS to alter substrate selectivity.
Externí odkaz:
https://doaj.org/article/65ce6f9e89b844d7adc627e3f70e0f01
Autor:
Jason Micklefield, Ying Zhuo, Katherine J Robins, Yingxin Zhang, Joanna K Fyans, Brian J. C. Law, Wei Li Thong, Abigail J Herbert
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
Thong, W L, Zhang, Y, Zhuo, Y, Robins, K J, Fyans, J K, Herbert, A J, Law, B J C & Micklefield, J 2021, ' Gene editing enables rapid engineering of complex antibiotic assembly lines ', Nature Communications, vol. 12, no. 1, 6872 . https://doi.org/10.1038/s41467-021-27139-1
Nature Communications
Thong, W L, Zhang, Y, Zhuo, Y, Robins, K J, Fyans, J K, Herbert, A J, Law, B J C & Micklefield, J 2021, ' Gene editing enables rapid engineering of complex antibiotic assembly lines ', Nature Communications, vol. 12, no. 1, 6872 . https://doi.org/10.1038/s41467-021-27139-1
Nature Communications
Re-engineering biosynthetic assembly lines, including nonribosomal peptide synthetases (NRPS) and related megasynthase enzymes, is a powerful route to new antibiotics and other bioactive natural products that are too complex for chemical synthesis. H
Autor:
Luke J. Stevenson, Katherine J. Robins, Janine K. Sharma, Elsie M. Williams, Abigail V. Sharrock, Adele K. Williamson, Vickery L. Arcus, Wayne M. Patrick, David F. Ackerley
Publikováno v:
Enzyme and Microbial Technology. 163:110153
DNA ligases are widely used in molecular biology to generate recombinant DNA. However, having evolved for nick-sealing, they are inefficient at catalysing the blunt-ended ligations that are critical to many biotechnological applications, including ne
Autor:
Maxinne Watchon, Amanda L. Wright, Holly I. Ahel, Katherine J. Robinson, Stuart K. Plenderleith, Andrea Kuriakose, Kristy C. Yuan, Angela S. Laird
Publikováno v:
Molecular Brain, Vol 17, Iss 1, Pp 1-15 (2024)
Abstract Machado-Joseph disease (MJD), also known as spinocerebellar ataxia type 3, is a fatal neurodegenerative disease that causes loss of balance and motor co-ordination, eventually leading to paralysis. It is caused by the autosomal dominant inhe
Externí odkaz:
https://doaj.org/article/6f50372d2c4746069222f52115dcf314
Autor:
Mark J. Calcott, Kelsi R. Hall, Katherine J. Robins, Ralf Schwörer, Rory F Little, Wayne M. Patrick, Janine N. Copp, Elsie M. Williams, Gary B. Evans, David F. Ackerley, Michelle H. Rich
Publikováno v:
eLife, Vol 9 (2020)
eLife
eLife
Selection for a promiscuous enzyme activity provides substantial opportunity for competition between endogenous and newly-encountered substrates to influence the evolutionary trajectory, an aspect that is often overlooked in laboratory directed evolu
Autor:
Wayne M. Patrick, Gary B. Evans, Michelle H. Rich, Mark J. Calcott, Elsie M. Williams, Janine N. Copp, Ralf Schwörer, David F. Ackerley, Katherine J. Robins, Kelsi R. Hall, Rory F Little
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5e133dc545fa5777ab82d64f15b1a42c
https://doi.org/10.7554/elife.59081.sa2
https://doi.org/10.7554/elife.59081.sa2
Autor:
Janine K, Sharma, Luke J, Stevenson, Katherine J, Robins, Adele K, Williamson, Wayne M, Patrick, David F, Ackerley
Publikováno v:
Methods in enzymology. 644
DNA ligases have numerous applications in molecular biology and biotechnology. However, many of these applications require the ligation of blunt-ended DNA termini, which is an inefficient activity for existing commercial ligases. To address this limi
Autor:
Mark J. Calcott, Janine N. Copp, David F. Ackerley, Rory F Little, Elsie M. Williams, Ralf Schwörer, Wayne M. Patrick, Gary B. Evans, Michelle H. Rich, Kelsi R. Hall, Katherine J. Robins
Selection for a promiscuous enzyme activity provides substantial opportunity for competition between endogenous and new substrates to influence the evolutionary trajectory, an aspect that has generally been overlooked in laboratory directed evolution
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::99db4428e8063149e1b82f99bf7d1e2c
https://doi.org/10.1101/2020.05.27.118489
https://doi.org/10.1101/2020.05.27.118489
Autor:
Luke J Stevenson, Adele Kim Williamson, Katherine J. Robins, Wayne M. Patrick, Janine K Sharma, David F. Ackerley
DNA ligases have numerous applications in molecular biology and biotechnology. However, many of these applications require the ligation of blunt-ended DNA termini, which is an inefficient activity for existing commercial ligases. To address this limi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::084cdc777e8fe39c5ee928d71af57488
https://doi.org/10.1016/bs.mie.2020.04.061
https://doi.org/10.1016/bs.mie.2020.04.061
Publikováno v:
Scientific Reports
The ability to rapidly, economically and accurately measure L-glutamine concentrations in biological samples is important for many areas of research, medicine or industry, however there is room for improvement on existing methods. We describe here ho