Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Katherine E. Brechun"'
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-11 (2024)
Abstract Background Antibiotic-based plasmid selection and maintenance is a core tool in molecular biology; however, while convenient, this strategy has numerous drawbacks for biological manufacturing. Overuse of antibiotics and antibiotic resistance
Externí odkaz:
https://doaj.org/article/5e5632d23db5472a8140ecf2af420a87
Autor:
Danlin Zhen, Vitali Borisenko, Katherine E. Brechun, Wouter D. Hoff, Andrew Woolley, Masato Kumauchi, Katja M. Arndt, Anna S. I. Jaikaran
Publikováno v:
Biochemistry. 58:2682-2694
We report the design and characterization of photoactive yellow protein (PYP)-blue fluorescent protein (mTagBFP) fusion constructs that permit the direct assay of reconstitution and function of the PYP domain. These constructs allow for in vivo testi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6843bca35f310eb40506907bdbfecb5
https://doi.org/10.1021/acs.biochem.9b00279
https://doi.org/10.1021/acs.biochem.9b00279
We have developed a genetic circuit in Escherichia coli that can be used to select for protein-protein interactions of different strengths by changing antibiotic concentrations in the media. The genetic circuit links protein-protein interaction stren
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e67911c9879f9c131218db40ceb1041
https://europepmc.org/articles/PMC6331253/
https://europepmc.org/articles/PMC6331253/
Publikováno v:
Current opinion in structural biology. 45
Photo-controlled or 'optogenetic' effectors interfacing with endogenous protein machinery allow the roles of endogenous proteins to be probed. There are two main approaches being used to develop optogenetic effectors: (i) caging strategies using phot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fa918a2c6f09c9b36332f65e86a22d4
https://pubmed.ncbi.nlm.nih.gov/27907886
https://pubmed.ncbi.nlm.nih.gov/27907886
Autor:
Katherine E. Brechun, Stacy-Anne Morgan, Yih-Yang Chen, Helen Y. Fan, G. Andrew Woolley, Anna S. I. Jaikaran
Publikováno v:
Biochemistry. 50:1226-1237
Photocontrolled transcription factors could be powerful tools for probing the roles of transcriptional processes in a variety of settings. Previously, we designed a photocontrolled DNA-binding protein based on a fusion between the bZIP region of GCN4
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d478569ba3aeabf0a946df128ab51e0b
https://doi.org/10.1021/bi101432p
https://doi.org/10.1021/bi101432p
Autor:
G. Andrew Woolley, Carrie-Lynn Keiski, Katja M. Arndt, Stefan A. Hoffmann, Yih Yang Chen, Katherine E. Brechun, David R. McMillen, Yongjoo B. Kim, Mostafizur Mazumder
Publikováno v:
Protein engineering, designselection : PEDS. 28(9)
Light-switchable proteins offer numerous opportunities as tools for manipulating biological systems with exceptional degrees of spatiotemporal control. Most designed light-switchable proteins currently in use have not been optimised using the randomi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::305d13a4f806152dfeb4b043fdebe38e
https://pubmed.ncbi.nlm.nih.gov/26245690
https://pubmed.ncbi.nlm.nih.gov/26245690