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Autor:
Kathaleen Golds, Melissa K. Carpenter, Joseph D. Gold, Pratima Kundu, Margaret S. Inokuma, Jerrod Denham, Chunhui Xu
Publikováno v:
Nature Biotechnology. 19:971-974
Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated