Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Katerina Afanasieva"'
Autor:
Andrei Sivolob, Katerina Afanasieva
Publikováno v:
ScienceRise: Biological Science, Vol 0, Iss 5(14), Pp 4-9 (2018)
Структура хроматину на найвищих рівнях його організації, залишаючись не до кінця зрозумілою, привертає велику увагу, оскільки лежить в
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40c34695571c83d144f610fca27931c7
https://doi.org/10.15587/2519-8025.2018.143734
https://doi.org/10.15587/2519-8025.2018.143734
Autor:
Katerina Afanasieva, Andrei Sivolob
Publikováno v:
Biophysical Chemistry. 238:1-7
The comet assay is a sensitive method to assess DNA damages in single cells. The approach consists of an analysis of electrophoretic migration of DNA from nucleoids obtained after cell lysis in a thin layer of agarose. Although the method is widely u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e0f9b28065b1e141b0262f51124b00d
https://doi.org/10.1016/j.bpc.2018.04.003
https://doi.org/10.1016/j.bpc.2018.04.003
Publikováno v:
ELECTROPHORESIS. 36:973-977
Single-cell gel electrophoresis, or the comet assay, is usually performed with nucleoids prepared after a lysis of either whole cells (more often) or isolated cell nuclei (rarely). Electrophoretic properties of the second type of nucleoids have never
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::013d7aafebd1ac9c64fb81dff1104462
https://doi.org/10.1002/elps.201400566
https://doi.org/10.1002/elps.201400566
Autor:
L. L. Lukash, Marianna Chopei, Anastasia Semenova, Andrei Sivolob, Alexandra Lozovik, Katerina Afanasieva
Publikováno v:
Biochemical and biophysical research communications. 483(1)
The loop domain organization of chromatin plays an important role in transcription regulation and thus may be assumed to vary in cells of different types. We investigated the kinetics of DNA loop migration during single cell gel electrophoresis (the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bab115b4216469f55cde588495f1eda9
https://pubmed.ncbi.nlm.nih.gov/28039056
https://pubmed.ncbi.nlm.nih.gov/28039056
Publikováno v:
Electrophoresis. 36(7-8)
Single-cell gel electrophoresis, or the comet assay, is usually performed with nucleoids prepared after a lysis of either whole cells (more often) or isolated cell nuclei (rarely). Electrophoretic properties of the second type of nucleoids have never
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8175fc55684e500c3d31ff8c10eacf4d
https://pubmed.ncbi.nlm.nih.gov/25631953
https://pubmed.ncbi.nlm.nih.gov/25631953
Publikováno v:
Electrophoresis. 31(3)
We investigated the mechanisms of DNA exit during single-cell gel electrophoresis (the comet assay) by measuring the kinetics of the comet tail formation. In the neutral comet assay, the rate of DNA exit was found to be dependent on the topological s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d04bdc41c85fa1c8ad1459ae57d43b2
https://pubmed.ncbi.nlm.nih.gov/20119958
https://pubmed.ncbi.nlm.nih.gov/20119958
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. (12):3237-3244
At higher order levels chromatin is organized into loops. This looping, which plays an important role in transcription regulation and other processes, remains poorly understood. We investigated the kinetics of DNA loop migration during single cell ge
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e8eb4c4435497cb67c5194a838de473