Cprp—An Unusual, Repetitive Protein Which Impacts Pleuromutilin Biosynthesis in the Basidiomycete Clitopilus passeckerianus

Autor: Kate M. J. de Mattos-Shipley, Gary D. Foster, Andy M. Bailey

Publikováno v: Frontiers in Fungal Biology, Vol 2 (2021)

Interrogation of an EST database for Clitopilus passeckerianus identified a putative homolog to the unusual stress response gene from yeast; ddr48, as being upregulated under pleuromutilin production conditions. Silencing of this gene, named cprp, pr

Externí odkaz: https://doaj.org/article/50dc0ad5786b411ea54ebab925d69628

Cladobotric Acids: Metabolites from Cultures of Cladobotryum sp., Semisynthetic Analogues and Antibacterial Activity

Autor: Trong-Tuan Dao, Katherine Williams, Kate M. J. de Mattos-Shipley, Zhongshu Song, Yuiko Takebayashi, Thomas J. Simpson, James Spencer, Andrew M. Bailey, Christine L. Willis

Publikováno v: Dao, T T, Williams, K, de Mattos-Shipley, K M J, Song, Z, Takebayashi, Y, Simpson, T J, Spencer, J, Bailey, A M & Willis, C L 2022, ' Cladobotric acids: Metabolites from cultures of Cladobotryum sp., semi-synthetic analogues and antibacterial activity ', Journal of Natural Products, vol. 85, no. 3, pp. 572-580 . https://doi.org/10.1021/acs.jnatprod.1c01063

Three new polyketide-derived natural products, cladobotric acids G–I (1–3), and six known metabolites (4, 5, 8–11) were isolated from fermentation of the fungus Cladobotryum sp. grown on rice. Their structures were elucidated by extensive spect

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a7e5b2521ebfde65846ed25cb388880
https://doi.org/10.1021/acs.jnatprod.1c01063

Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Aspergillus oryzae as a Heterologous Host

Autor: Kate M J, de Mattos-Shipley, Colin M, Lazarus, Katherine, Williams

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2489

A suite of molecular techniques have been developed in recent decades, which allow gene clusters coding for the biosynthesis of fungal natural products to be investigated and characterized in great detail. Many of these involve the manipulation of th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::917762b5c0d8f906d4d27ffca3f18c07
https://pubmed.ncbi.nlm.nih.gov/35524043

Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Aspergillus oryzae as a Heterologous Host

Autor: Kate M. J. de Mattos-Shipley, Colin M. Lazarus, Katherine Williams

Publikováno v: Methods in Molecular Biology ISBN: 9781071622728

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8dd0d57c74e7b8c65934c710fe63edcb
https://doi.org/10.1007/978-1-0716-2273-5_2

Cleaning the Cellular Factory–Deletion of McrA in Aspergillus oryzae NSAR1 and the Generation of a Novel Kojic Acid Deficient Strain for Cleaner Heterologous Production of Secondary Metabolites

Autor: Katherine Williams, Trong Tuan Dao, Colin M. Lazarus, Christine L. Willis, Kate M. J. de Mattos-Shipley, Andy M. Bailey, Marija K Zacharova, Ian M. Prosser

Publikováno v: Dao, T T, De Mattos-Shipley, K M, Prosser, I M, Williams, K, Zacharova, M K, Lazarus, C M, Willis, C L & Bailey, A M 2021, ' Cleaning the Cellular Factory : Deletion of McrA in Aspergillus oryzae NSAR1 and the generation of a novel kojic acid deficient strain for cleaner heterologous production of secondary metabolites ', Frontiers in Fungal Biology, vol. 2, no. 632542 . https://doi.org/10.3389/ffunb.2021.632542

The use of filamentous fungi as cellular factories, where natural product pathways can be refactored and expressed in a host strain, continues to aid the field of natural product discovery. Much work has been done to develop host strains which are ge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d285edc027df24b10cb4da54588a90c3
https://doi.org/10.3389/ffunb.2021.632542