Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Katarina S. Midelfort"'
Autor:
Seungil Han, Katarina S. Midelfort, Marie Anderson, Jeanne S. Chang, Jeremy Minshull, Alan Villalobos, Michael J. Karmilowicz, Rajesh Kumar, Daniel K. Gehlhaar, Kevin McConnell, Anil Mistry, John W. Wong, Sridhar Govindarajan
Publikováno v:
Protein Engineering Design and Selection. 26:25-33
Several protein engineering approaches were combined to optimize the selectivity and activity of Vibrio fluvialis aminotransferase (Vfat) for the synthesis of (3S,5R)-ethyl 3-amino-5-methyloctanoate; a key intermediate in the synthesis of imagabalin,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9bc6d43d43bf4fd4f52a74ec898a7e9
https://doi.org/10.1093/protein/gzs065
https://doi.org/10.1093/protein/gzs065
Autor:
John W. Wong, Thomas S. Moody, Jeremy Steflik, Maria S. Brown, Owen W. Gooding, Gjalt W. Huisman, David Mangan, Carlos A. Martinez, Jack Liang, Michael Paul Burns, Adam A. Smogowicz, Katarina S. Midelfort
Publikováno v:
Practical Methods for Biocatalysis and Biotransformations 2
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7e5c3172496feec5a814a3e282c19870
https://doi.org/10.1002/9781119943426.ch4
https://doi.org/10.1002/9781119943426.ch4
Autor:
Katarina S. Midelfort, K. Dane Wittrup
Publikováno v:
Protein Science. 15:324-334
A mutational analysis of the femtomolar-affinity anti-fluorescein antibody 4M5.3, compared to its wild-type progenitor, 4-4-20, indicates both context-dependent and -independent mutations are responsible for the 1800-fold affinity improvement. 4M5.3
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5dae3135aac66b1fc00ee832c217a0c9
https://doi.org/10.1110/ps.051842406
https://doi.org/10.1110/ps.051842406
Publikováno v:
Proceedings of the National Academy of Sciences. 97:10701-10705
Single-chain antibody mutants have been evolvedin vitrowith antigen-binding equilibrium dissociation constantKd= 48 fM and slower dissociation kinetics (half-time > 5 days) than those for the streptavidin–biotin complex. These mutants possess the h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf5c756889834a3f5936f96847670f60
https://doi.org/10.1073/pnas.170297297
https://doi.org/10.1073/pnas.170297297