Zobrazeno 1 - 10
of 34
pro vyhledávání: '"Karsten Kuhn"'
Autor:
Ian Pike, Jiaming Li, Thomas M. Roberts, Steven P. Gygi, Karsten Kuhn, John C. Rogers, Joao A. Paulo, Zhenying Cai, Ryan Bomgarden
Publikováno v:
J Proteome Res
The development of the TMTpro-16plex series expanded the breadth of commercial isobaric tagging reagents by nearly 50% over classic TMT-11plex. In addition to the described 16plex reagents, the proline-based TMTpro molecule can accommodate two additi
Autor:
Stephan Jung, Dennis Friedel, Ines Heiland, Tobias Bausbacher, Christiane A. Opitz, Stefan Selzer, Ian Pike, Andreas von Deimling, Antje Wick, Karsten Kuhn, Suraj Sharma, Verena Panitz, Irada Pflüger, Sandra Schulz, Wolfgang Wick, Philipp Vollmuth, Sasa Koncarevic, Philipp Sievers, Saskia Trump, Stefan Schmidt, Carsten Hopf, Felix Sahm, Peter Schulz-Knappe, Michael Platten, V. M. Farztdinov, Pauline Pfänder, Ina Jürgenson, Ahmed Sadik
Publikováno v:
Theranostics
Tryptophan (Trp)-catabolic enzymes (TCEs) produce metabolites that activate the aryl hydrocarbon receptor (AHR) and promote tumor progression and immunosuppression in glioblastoma. As therapies targeting TCEs or AHR become available, a better underst
Autor:
Premchendar Nandhikonda, Joao A. Paulo, Rosa Viner, Aaron M. Robitaille, Ian Pike, Steven P. Gygi, John C. Rogers, Chris Etienne, Laura Pontano Vaites, Jonathan G. Van Vranken, Andrew Hugin Thompson, Karsten Kuhn, Devin K. Schweppe, Jiaming Li, Edward L. Huttlin, Ryan Bomgarden
Publikováno v:
Nat Methods
Isobaric labeling empowers proteome-wide expression measurements simultaneously across multiple samples. Here an expanded set of 16 isobaric reagents based on an isobutyl-proline immonium ion reporter structure (TMTpro) is presented. These reagents h
Autor:
Stephan Jung, Ian Pike, Stefan Selzer, Petra Penning, Karsten Kuhn, Claudia Höhle, Harald Legner, Stefan Kienle, Andrew J. Thompson, Roxana Martinez-Pinna, Sasa Koncarevic, Peter Schmid, Antje Berfelde, V. M. Farztdinov, Gitte Böhm, Nikolai Wölmer
Publikováno v:
Analytical Chemistry. 91:15941-15950
The design and synthesis of a proline-based reporter isobaric Tandem Mass Tag structure (TMTpro) is presented. An analysis is made of the performance of the new TMTpro tags in comparison with the current commercially available dimethylpiperidine-repo
Autor:
Malcolm Ward, Ulla Rüetschi, Mikko Hölttä, Oskar Hansson, Ian Pike, Henrik Zetterberg, Karsten Kuhn, Lennart Minthon, Johan Gobom, Jessica Holmén-Larsson, Kaj Blennow
Publikováno v:
Journal of Proteome Research. 14:654-663
Many disease processes in the brain are reflected in the protein composition of the cerebrospinal fluid (CSF). In addition to proteins, CSF also contains a large number of endogenous peptides whose potential as disease biomarkers largely remains to b
Autor:
Lily Ting, John C. Rogers, Justin D. Blethrow, Robert A. Grothe, Mark P. Jedrychowski, Steven P. Gygi, Wilhelm Haas, Graeme C. McAlister, Edward L. Huttlin, Ian Pike, Karsten Kuhn
Publikováno v:
Analytical Chemistry. 84:7469-7478
Quantitative mass spectrometry methods offer near-comprehensive proteome coverage; however, these methods still suffer with regards to sample throughput. Multiplex quantitation via isobaric chemical tags (e.g., TMT and iTRAQ) provides an avenue for m
Autor:
Peter van Ulsen, Thorsten Prinz, Peter Schmid, Harald Legner, Jan Tommassen, Karsten Kuhn, Christian Baumann
Publikováno v:
Journal of Proteomics, 1771-1781. Elsevier
ISSUE=7;STARTPAGE=1771;ENDPAGE=1781;ISSN=1874-3919;TITLE=Journal of Proteomics
van Ulsen, J P, Kuhn, K, Prinz, T, Legner, H, Schmid, P, Baumann, C & Tommassen, J 2009, ' Identification of proteins of Neisseria meningitidis induced under iron-limiting conditions using the isobaric tandem mass tag (TMT) labeling approach. ', Journal of Proteomics, no. 7, pp. 1771-1781 . https://doi.org/10.1002/pmic.200800642
ISSUE=7;STARTPAGE=1771;ENDPAGE=1781;ISSN=1874-3919;TITLE=Journal of Proteomics
van Ulsen, J P, Kuhn, K, Prinz, T, Legner, H, Schmid, P, Baumann, C & Tommassen, J 2009, ' Identification of proteins of Neisseria meningitidis induced under iron-limiting conditions using the isobaric tandem mass tag (TMT) labeling approach. ', Journal of Proteomics, no. 7, pp. 1771-1781 . https://doi.org/10.1002/pmic.200800642
Isobaric labeling reagents such as Tandem Mass Tags (TMT®) enable the genome-wide quantification of protein expression levels under different conditions using a gel-free MS/MS-based approach. Here, we applied a TMTduplex approach with two isobaric t
Autor:
Virginie Licker, Jean-Charles Sanchez, Denis F. Hochstrasser, Loïc Dayon, Natacha Turck, Alexandre Hainard, Pierre Burkhard, Karsten Kuhn
Publikováno v:
Analytical Chemistry, Vol. 80, No 8 (2008) pp. 2921-31
A new 6-plex isobaric mass tagging technology is presented, and proof of principle studies are carried out using standard protein mixtures and human cerebrospinal fluid (CSF) samples. The Tandem Mass Tags (TMT) comprise a set of structurally identica
Autor:
Jörg Müller, Josef Schwarz, Jürgen Schäfer, Sylvane Desrivières, Jutta Korder, Melanie Gläser, Karsten Kuhn, Thorsten Prinz, Michael Sonnentag, Bernd Groner, Christian Hamon, Thomas Neumann, Nahomi Castro-Palomino Laria
Publikováno v:
PROTEOMICS. 7:2019-2037
The progression of stem cells to proliferating progenitor cells and finally to a quiescent differentiated state is a hallmark of organ development. This process proceeds through distinct steps and is regulated through cell-cell interactions and by sy
Autor:
Ian Pike, Petra Prefot, Karsten Kuhn, Stefan Selzer, Andrew Hugin Thompson, Gitte Böhm, Vikram Mitra, Stephan Jung, David Britton
Publikováno v:
Journal of proteome research. 14(6)
We present a novel tandem mass tag solid-phase amino labeling (TMT-SPAL) protocol using reversible immobilization of peptides onto octadecyl-derivatized (C18) solid supports. This method can reduce the number of steps required in complex protocols, s