Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Karl-Ludwig Schimz"'
Autor:
Eve Willery, Françoise Jacob-Dubuisson, Hans-Georg Koch, Nina Chevalier, Camille Locht, Karl-Ludwig Schimz, Michael Moser, Matthias Müller
Publikováno v:
Microbial Physiology. 8:7-18
Filamentous haemagglutinin (FHA) is the major adhesin of Bordetella pertussis, the whooping cough agent. FHA is synthesized as a 367-kDa precursor harbouring a remarkably long signal peptide with an N-terminal extension that is conserved among relate
Publikováno v:
The journal of biological chemistry 277, 5715-5718 (2002). doi:10.1074/jbc.C100683200
The integration of the polytopic membrane protein YidC into the inner membrane of Escherichia coli was analyzed employing an in vitro system. Upon integration of in vitro synthesized YidC, a 42-kDa membrane protected fragment was detected, which coul
Autor:
Hans Jürgen Plattner, Haiming Li, Michael Kieß, Jochen Meens, Hans Diekmann, Karl-Ludwig Schimz
Publikováno v:
Biotechnology Letters. 22:1203-1209
A new chitinase gene, chi92, encoding the largest known chitinase from Streptomyces olivaceoviridisATCC 11238 was sequenced by means of different PCR-methods. The cloned gene was expressed in E. coliand the recombinant protein could be detected by We
Autor:
Karl-Ludwig Schimz, Matthias Müller, Juan MacFarlane, Bernd Mechler, Christoph Neumann-Haefelin, Thomas Hengelage, Hedda K. Hoffschulte, Hans-Georg Koch
Publikováno v:
Molecular Biology of the Cell. 10:2163-2173
The molecular requirements for the translocation of secretory proteins across, and the integration of membrane proteins into, the plasma membrane of Escherichia coli were compared. This was achieved in a novel cell-free system from E. coliwhich, by e
Autor:
Maria-Regina Kula, Martina Pohl, Holger Breithaupt, Wolfram Bönigk, Karl-Ludwig Schimz, Petra Heim
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 6:315-332
The gene encoding for (R)-hydroxynitrile lyase ((R)-HNL) from Linum usitatissimum has been cloned by polymerase chain reaction using 3′,5′-RACE (rapid amplification of cDNA ends). The resulting clone contained an open reading frame of 1266 bp cor
Publikováno v:
FEBS Letters. 362:29-33
A cell-free protein translocation system derived exclusively from a Gram-positive bacterium is described here for the first time. Highly efficient in vitro synthesis of plasmid encoded preprolipase of Staphylococcus hyicus is accomplished by coupled
Publikováno v:
Journal of General Microbiology. 139:2833-2840
The filamentous fungus Botrytis cinerea was found to produce four 1,3-β-glucanases (Glu I, II, III, IV). These enzymes were visualized by activity-staining after separation by native polyacrylamide gel electrophoresis (PAGE). During growth on glucos
Publikováno v:
Applied and Environmental Microbiology. 58:3347-3354
During growth on glucose, Botrytis cinerea produced extracellular β-(1,3)(1,6)- d -glucan (cinerean), which formed an adhering capsule and slime. After glucose was exhausted from the medium, cinereanase activity increased from B. cinerea was found t
Publikováno v:
FEBS letters. 425(1)
Oxazolidinones are a promising new class of synthetic antibiotics active against multidrug-resistant Gram-positive bacteria. To elucidate their mode of action, the effect of DuP 721 on individual steps of protein translation was studied. The drug doe
Publikováno v:
European Journal of Biochemistry. 59:545-559
1. The existence of two different D-glucose-6-phosphate dehydrogenases in Pseudomonas fluorescens has been demonstrated. Based on their different specificity and their different metabolic regulation one enzyme is appointed to the Entner-Doudoroff pat