Zobrazeno 1 - 10
of 90
pro vyhledávání: '"Karl A. Wilson"'
Publikováno v:
Plant Physiology and Biochemistry. 104:242-249
During soybean (Glycine max (L.) Merrill) seed development, protease C1, the proteolytic enzyme that initiates breakdown of the storage globulins β-conglycinin and glycinin at acidic pH, is present in the protein storage vacuoles (PSVs), the same su
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5706deb9d200ce1c3243da7e08d5553
https://doi.org/10.1016/j.plaphy.2016.03.031
https://doi.org/10.1016/j.plaphy.2016.03.031
Autor:
Karl A. Wilson, Anna L. Tan-Wilson
Publikováno v:
Plant Physiology and Biochemistry. 96:301-310
The 7S and 11S globulins of peanuts are subjected to proteolysis two days after seed imbibition, with Ara h 1 and the arachin acidic chains being among the first storage proteins to be mobilized. Proteolytic activity was greatest at pH 2.6-3 and is i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6d6779337286f3bd4a42fc5350d5657
https://doi.org/10.1016/j.plaphy.2015.08.008
https://doi.org/10.1016/j.plaphy.2015.08.008
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1626
The technique described here, transfer zymography, was developed to overcome two limitations of conventional zymography. When proteolytic enzymes are resolved by nonreducing SDS-PAGE into a polyacrylamide gel with copolymerized protein substrate, the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::33c2c22d3901bc9183e1c2af14a296a7
https://pubmed.ncbi.nlm.nih.gov/28608218
https://pubmed.ncbi.nlm.nih.gov/28608218
Autor:
Anna L. Tan-Wilson, Karl A. Wilson
Publikováno v:
Journal of plant physiology.
Legume species differ in whether or not the 7S globulins stored in seeds undergo proteolytic processing during seed development, while preserving the bicupin structure and trimeric assembly necessary for accumulation and packing into protein storage
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::542d2135177f1cbb9c245abbd7483dc1
https://pubmed.ncbi.nlm.nih.gov/29609123
https://pubmed.ncbi.nlm.nih.gov/29609123
Publikováno v:
Zymography ISBN: 9781493971091
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0f2637ef7e07b58185cf1bb745ec6f32
https://doi.org/10.1007/978-1-4939-7111-4_24
https://doi.org/10.1007/978-1-4939-7111-4_24
Autor:
Anna L. Tan-Wilson, Karl A. Wilson
Publikováno v:
Biochemistry and Molecular Biology Education. 41:79-86
Mass spectrometry (MS) has become an important tool in studying biological systems. One application is the identification of proteins and peptides by the matching of peptide and peptide fragment masses to the sequences of proteins in protein sequence
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e3e85caadcd162c822f24ab951037bfb
https://doi.org/10.1002/bmb.20659
https://doi.org/10.1002/bmb.20659
Autor:
Anna L. Tan-Wilson, Karl A. Wilson
Publikováno v:
Physiologia Plantarum. 145:140-153
The mobilization of seed storage proteins upon seed imbibition and germination is a crucial process in the establishment of the seedling. Storage proteins fold compactly, presenting only a few vulnerable regions for initial proteolytic digestion. Evo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29439993548869753ecbc5982ed47aae
https://doi.org/10.1111/j.1399-3054.2011.01535.x
https://doi.org/10.1111/j.1399-3054.2011.01535.x
Publikováno v:
Analytical Biochemistry. 411:277-283
Zymography detects and characterizes proteolytic enzymes by electrophoresis of protease-containing samples into a nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel containing a copolymerized protein substrate. The u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::339b3d052b7bea40403fe3d883911d76
https://doi.org/10.1016/j.ab.2011.01.015
https://doi.org/10.1016/j.ab.2011.01.015
Publikováno v:
Journal of Experimental Botany. 58:1059-1070
Soybean protease C1 (EC 3.4.21.25), the subtilisin-like serine protease that initiates the proteolysis of seed storage proteins in germinating soybean [Glycine max (L.) Merrill], was localized to the protein storage vacuoles of parenchyma cells in th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99895deb748336442490c35141d07894
https://doi.org/10.1093/jxb/erl267
https://doi.org/10.1093/jxb/erl267
Publikováno v:
Biochemistry. Biokhimiia. 80(10)
Legumin and vicilin are two-domain seed storage globulins similar in primary and higher order structures of their domains to single-domain plant germins as well as to the domains of two-domain and single-domain bacterial oxalate decarboxylases. Indep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b0b805f6fec5b222748b0cf15cb0032
https://pubmed.ncbi.nlm.nih.gov/26567580
https://pubmed.ncbi.nlm.nih.gov/26567580