Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Karina Jouravleva"'
Publikováno v:
STAR Protocols, Vol 4, Iss 2, Pp 102336- (2023)
Summary: Binding affinity quantitatively describes the strength of a molecular interaction and is reported by the equilibrium dissociation constant (KD). Here, we present a protocol to measure KD of mammalian microRNA-loaded Argonaute2 protein by dou
Externí odkaz:
https://doaj.org/article/6d1f7c2a85a04687ac738e1d29345a05
Autor:
Carlas S. Smith, Karina Jouravleva, Maximiliaan Huisman, Samson M. Jolly, Phillip D. Zamore, David Grunwald
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
Analysis of single-molecule binding assays still requires substantial manual user intervention. Here, the authors present a pipeline for rapid, automated analysis of co-localization single-molecule spectroscopy images, with a modular user interface t
Externí odkaz:
https://doaj.org/article/843e839540ba4dd28ca6c75bbc68d526
Autor:
Karina Jouravleva, Dmitrij Golovenko, Gabriel Demo, Robert C. Dutcher, Traci M. Tanaka Hall, Phillip D. Zamore, Andrei A. Korostelev
Publikováno v:
Molecular Cell. 82:4049-4063.e6
SUMMARYIn animals and plants, Dicer enzymes collaborate with double-stranded RNA-binding proteins to convert precursor-microRNAs (pre-miRNAs) into miRNA duplexes. We report six cryo-EM structures of Drosophila Dicer-1 and its partner Loqs-PB. The str
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b8b2b12fe06d6cacd4e7db621b1a40e
https://doi.org/10.1016/j.molcel.2022.09.002
https://doi.org/10.1016/j.molcel.2022.09.002
Autor:
Benjamin Ober-Reynolds, Winston R. Becker, Karina Jouravleva, Samson M. Jolly, Phillip D. Zamore, William J. Greenleaf
Publikováno v:
Mol Cell
Argonautes are nucleic acid-guided proteins that perform numerous cellular functions across all domains of life. Little is known about how distinct evolutionary pressures have shaped each Argonaute's biophysical properties. We applied high-throughput
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06a6c38a0e5056ec93941fc85692dbcd
https://doi.org/10.1016/j.molcel.2022.02.026
https://doi.org/10.1016/j.molcel.2022.02.026
Publikováno v:
Cell Reports Methods. 2:100185
RNA Bind-n-Seq (RBNS) is a cost-effective, high-throughput method capable of identifying the sequence preferences of RNA-binding proteins and of qualitatively defining relative dissociation constants. Although RBNS is often described as an unbiased m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f5099f5036157b7466e8106fc93a1a2
https://doi.org/10.1016/j.crmeth.2022.100185
https://doi.org/10.1016/j.crmeth.2022.100185
Autor:
Yongjin Lee, Karina Jouravleva, Paul Albosta, Phillip D. Zamore, Cansu Colpan, Katharine Cecchini, Joel Vega-Badillo, Ildar Gainetdinov, Deniz M. Ozata
In animals, piRNAs, siRNAs, and miRNAs silence transposons, fight viral infections, and regulate gene expression. piRNA biogenesis concludes with 3′ terminal trimming and 2′-O-methylation. Both trimming and methylation influence piRNA stability.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8ec13880fe6457b03036231b384a472a
https://doi.org/10.1101/2020.09.08.287979
https://doi.org/10.1101/2020.09.08.287979
Autor:
Samson M. Jolly, Ildar Gainetdinov, Karina Jouravleva, Han Zhang, Lara Strittmatter, Gregory M. Hendricks, Avantika Dhabaria, Beatrix Ueberheide, Phillip D. Zamore
Argonaute proteins use nucleic acid guides to protect organisms against transposons and viruses. In the eubacterium Thermus thermophilus, the DNA-guided Argonaute TtAgo defends against transformation by DNA plasmids. Here, we report that TtAgo also p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a031234636c3ad0e9b221289a076aa2
Autor:
Ildar Gainetdinov, Phillip D. Zamore, Joel Vega-Badillo, Katharine Cecchini, Amena Arif, Yongjin Lee, Paul Albosta, Deniz M. Ozata, Cansu Colpan, Karina Jouravleva
Publikováno v:
Mol Cell
Summary In animals, PIWI-interacting RNAs (piRNAs) silence transposons, fight viral infections, and regulate gene expression. piRNA biogenesis concludes with 3′ terminal trimming and 2′-O-methylation. Both trimming and methylation influence piRNA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f91fa9ea93de266dda50ebe3ca48def6
https://doi.org/10.1016/j.molcel.2021.09.012
https://doi.org/10.1016/j.molcel.2021.09.012
Autor:
Phillip D. Zamore, Benjamin Ober-Reynolds, Samson M. Jolly, William J. Greenleaf, Winston R. Becker, Karina Jouravleva
Publikováno v:
Mol Cell
Summary Argonaute proteins loaded with microRNAs (miRNAs) or small interfering RNAs (siRNAs) form the RNA-induced silencing complex (RISC), which represses target RNA expression. Predicting the biological targets, specificity, and efficiency of both
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34cde4ffa1d4000073627541ed78ca40
https://europepmc.org/articles/PMC6823844/
https://europepmc.org/articles/PMC6823844/
Autor:
Phillip D. Zamore, Karina Jouravleva
Publikováno v:
Nature cell biology. 21(4)
MicroRNAs (miRNAs) repress target mRNAs, often with exquisite tissue specificity. Wang et al. exploit the specific expression of miRNAs to regulate guide production for Cas9. Their method enables novel strategies to simultaneously measure the activit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::219966053a99b4e52b244313a593fb41
https://pubmed.ncbi.nlm.nih.gov/30936471
https://pubmed.ncbi.nlm.nih.gov/30936471