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pro vyhledávání: '"Karina A. Baksh"'
Publikováno v:
Journal of Biological Chemistry. 299:102785
In Helicobacter pylori, the nickel-responsive NikR transcription factor plays a key role in regulating intracellular nickel concentrations, which is an essential process for survival of this pathogen in the acidic human stomach. Nickel binding to H.
Publikováno v:
The Journal of Biological Chemistry
Nickel is essential for the survival of the pathogenic bacteria Helicobacter pylori in the fluctuating pH of the human stomach. Due to its inherent toxicity and limited availability, nickel homeostasis is maintained through a network of pathways that
Autor:
Karina A. Baksh, Deborah B. Zamble
Publikováno v:
The Journal of biological chemistry. 295(6)
Many transition metals are essential trace nutrients for living organisms, but they are also cytotoxic in high concentrations. Bacteria maintain the delicate balance between metal starvation and toxicity through a complex network of metal homeostasis
Publikováno v:
Protein Expression and Purification. 167:105541
Polyhistidine tags (His-tags) are commonly employed in protein purification strategies due to the high affinity and specificity for metal-NTA columns, the relative simplicity of such protocols, and the assumption that His-tags do not affect the nativ