Zobrazeno 1 - 10
of 29
pro vyhledávání: '"Karin Ippen-Ihler"'
Autor:
Seema Endley, Pushpa Kathir, Kesmanee Maneewannakul, Jan Manchak, Deanna Moore, Laura S. Frost, Karin Ippen-Ihler
Publikováno v:
Molecular Microbiology. 22:197-205
A derivative of the F plasmid, pOX38-tra715, expresses the entire F tra operon from a foreign promoter (PT7) derived from phage T7. A series of plasmids related to pOX38-tra715 were constructed which carry either deletion mutations or point mutations
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31720023b8d905ae150774e24cbc1e37
https://doi.org/10.1046/j.1365-2958.1996.00087.x
https://doi.org/10.1046/j.1365-2958.1996.00087.x
Publikováno v:
Journal of Bacteriology. 177:2957-2964
Acetylation of F-pilin subunits has previously been shown to depend upon expression of the F plasmid transfer operon gene traX. To assess the requirement for pilin acetylation in conjugative transfer of F, we constructed traX::kan insertion mutations
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::640dc18ba5319b4916f39415f257e6d2
https://doi.org/10.1128/jb.177.11.2957-2964.1995
https://doi.org/10.1128/jb.177.11.2957-2964.1995
Autor:
Deanna Moore, L S Frost, C. M. Hamilton, Yoav Mintz, Kesmanee Maneewannakul, Karin Ippen-Ihler
Publikováno v:
Journal of Bacteriology. 175:1375-1383
The Escherichia coli F plasmid gene required for amino-terminal acetylation of F-pilin subunits was identified. Using Western blots (immunoblots), we assayed the reaction of monoclonal antibodies with F-pilin polypeptides in inner membrane preparatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f09f910856d286866796fde5b6e496ae
https://doi.org/10.1128/jb.175.5.1375-1383.1993
https://doi.org/10.1128/jb.175.5.1375-1383.1993
Publikováno v:
Journal of Bacteriology. 175:1384-1391
Transfer of the Escherichia coli fertility plasmid, F, is dependent on expression of F pili. Synthesis of F-pilin subunits is known to involve three F plasmid transfer (tra) region products: traA encodes the 13-kDa precursor protein, TraQ permits thi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac63c3e59f3f971a77eb62e9142c33d8
https://doi.org/10.1128/jb.175.5.1384-1391.1993
https://doi.org/10.1128/jb.175.5.1384-1391.1993
Publikováno v:
Journal of Molecular Biology. 225:299-311
The product of the F plasmid transfer gene, traN, is thought to be required for the formation of stable mating aggregates during F-directed conjugation. By testing chimeric plasmids that express F transfer region segments for complementation of F lac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51f9576211986786b1f3e36f90585298
https://doi.org/10.1016/0022-2836(92)90923-8
https://doi.org/10.1016/0022-2836(92)90923-8
Publikováno v:
Journal of bacteriology. 178(13)
F-pilin maturation and translocation result in the cleavage of a 51-amino-acid leader sequence from propilin and require LepB and TraQ but not the SecA-SecY secretion pathway. The unusual propilin leader peptide and the dependence of its cleavage on
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5810389a1c70ca6bac76d472c1be2a8
https://pubmed.ncbi.nlm.nih.gov/8682776
https://pubmed.ncbi.nlm.nih.gov/8682776
Publikováno v:
Plasmid. 31(3)
We describe a set of plasmid vectors that are very useful for cloning, expressing, mutagenizing, deleting, and sequencing DNA fragments. A strategy for using one (pKSM717) to obtain mutant protein products that contain deletions of N-terminal amino a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68d4e57f4ca0b4c76cf90f6681eb8a5b
https://pubmed.ncbi.nlm.nih.gov/8058823
https://pubmed.ncbi.nlm.nih.gov/8058823
Publikováno v:
Journal of bacteriology. 175(5)
F plasmid derivatives carrying kan insertion mutations in the transfer region genes traR, trbJ, and trbH were constructed. Standard tests indicated that these loci are not essential for F pilus production or F transfer among Escherichia coli K-12 hos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32c446e5acccb214539d802cb4c4c2e1
https://pubmed.ncbi.nlm.nih.gov/8444814
https://pubmed.ncbi.nlm.nih.gov/8444814
Autor:
Ronald A. Skurray, Karin Ippen-Ihler
Publikováno v:
Bacterial Conjugation ISBN: 9780306443763
The bacterial conjugation system encoded by the F sex factor was the first to be described and subjected to detailed genetic and molecular analysis (for recent reviews see 88 and 167, and for earlier reviews and articles see Chapter 1 of this volume)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9986059ca933feb958d975bbd8f602f6
https://doi.org/10.1007/978-1-4757-9357-4_2
https://doi.org/10.1007/978-1-4757-9357-4_2
Publikováno v:
Molecular microbiology. 6(20)
We constructed derivatives of the Escherichia coli conjugative plasmid F that carry altered sequences in place of the major transfer operon promoter, PY. Replacement of PY with a promoter-deficient sequence resulted in a transfer-deficient, F-pilus-s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d70e21748aae4e87bf8c6d6681f5156
https://pubmed.ncbi.nlm.nih.gov/1479888
https://pubmed.ncbi.nlm.nih.gov/1479888