Resource-aware construct design in mammalian cells

Autor: Roberto Di Blasi, Mara Pisani, Fabiana Tedeschi, Masue M. Marbiah, Karen Polizzi, Simone Furini, Velia Siciliano, Francesca Ceroni

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-10 (2023)

Abstract Resource competition can be the cause of unintended coupling between co-expressed genetic constructs. Here we report the quantification of the resource load imposed by different mammalian genetic components and identify construct designs wit

Externí odkaz: https://doaj.org/article/e31e743ce5614e45973f217d7be6ea1b

Polymer Encapsulation of Bacterial Biosensors Enables Coculture with Mammalian Cells

Autor: Ignacio Moya-Ramírez, Pavlos Kotidis, Masue Marbiah, Juhyun Kim, Cleo Kontoravdi, Karen Polizzi

Publikováno v: ACS Synthetic Biology. 11:1303-1312

Coexistence of different populations of cells and isolation of tasks can provide enhanced robustness and adaptability or impart new functionalities to a culture. However, generating stable cocultures involving cells with vastly different growth rates

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5bc18ed962fced8211d1aae099a86695
https://doi.org/10.1021/acssynbio.1c00577

The sound of silence: Transgene silencing in mammalian cell engineering

Autor: Alan Cabrera, Hailey I. Edelstein, Fokion Glykofrydis, Kasey S. Love, Sebastian Palacios, Josh Tycko, Meng Zhang, Sarah Lensch, Cara E. Shields, Mark Livingston, Ron Weiss, Huimin Zhao, Karmella A. Haynes, Leonardo Morsut, Yvonne Y. Chen, Ahmad S. Khalil, Wilson W. Wong, James J. Collins, Susan J. Rosser, Karen Polizzi, Michael B. Elowitz, Martin Fussenegger, Isaac B. Hilton, Joshua N. Leonard, Lacramioara Bintu, Kate E. Galloway, Tara L. Deans

Publikováno v: Cabrera, A, Edelstein, H I, Glykofrydis, F, Love, K S, Palacios, S, Tycko, J, Zhang, M, Lensch, S, Shields, C E, Livingston, M, Weiss, R, Zhao, H, Haynes, K A, Morsut, L, Chen, Y Y, Khalil, A S, Wong, W W, Collins, J J, Rosser, S J, Polizzi, K, Elowitz, M B, Fussenegger, M, Hilton, I B, Leonard, J N, Bintu, L, Galloway, K E & Deans, T L 2022, ' The sound of silence : Transgene silencing in mammalian cell engineering ', Cell Systems, vol. 13, no. 12, pp. 950-973 . https://doi.org/10.1016/j.cels.2022.11.005

To elucidate principles operating in native biological systems and to develop novel biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within native transcriptional networks. The utility of synthetic gene circuits

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4cf63d8a091cd74103de6c21ce1c1f69
https://pubmed.ncbi.nlm.nih.gov/36549273

FRONT MATTER

Autor: Geoff Baldwin, Travis Bayer, Robert Dickinson, Tom Ellis, Paul S Freemont, Richard I Kitney, Karen Polizzi, Guy-Bart Stan

Publikováno v: Synthetic Biology — A Primer. :i-xiv

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a0956a88d747b602f4e3f586dc729551
https://doi.org/10.1142/9781783268801_fmatter

BACK MATTER

Autor: Geoff Baldwin, Travis Bayer, Robert Dickinson, Tom Ellis, Paul S Freemont, Richard I Kitney, Karen Polizzi, Guy-Bart Stan

Publikováno v: Synthetic Biology — A Primer. :151-179

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0c87555e1e9acef1e580e66a5aa12659
https://doi.org/10.1142/9781848168640_bmatter