Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Karen Meyer-Arendt"'
Autor:
Karen Meyer-Arendt, William M. Old, Katheryn A. Resing, Kutralanathan Renganathan, Stephane Houel, Brian Eichelberger, Natalie G. Ahn
Publikováno v:
Journal of Proteome Research
When analyzing proteins in complex samples using tandem mass spectrometry of peptides generated by proteolysis, the inference of proteins can be ambiguous, even with well-validated peptides. Unresolved questions include whether to show all possible p
Autor:
Karen Meyer-Arendt, William M. Old, Natalie G. Ahn, Kutralanathan Renganathan, Stephane Houel, Robert Abernathy
Publikováno v:
Journal of Proteome Research. 9:4152-4160
A complicating factor for protein identification within complex mixtures by LC/MS/MS is the problem of "chimera" spectra, where two or more precursor ions with similar mass and retention time are co-sequenced by MS/MS. Chimera spectra show reduced sc
Autor:
Natalie G. Ahn, Krzysztof J. Cios, Katheryn A. Resing, Kevin G. Pierce, Chia-Yu Yen, Karen Meyer-Arendt, Robert Brown, Shaojun Sun, Brian Eichelberger, William M. Old
Publikováno v:
Molecular & Cellular Proteomics. 6:1-17
A major limitation in identifying peptides from complex mixtures by shotgun proteomics is the ability of search programs to accurately assign peptide sequences using mass spectrometric fragmentation spectra (MS/MS spectra). Manual analysis is used to
Autor:
Karen Meyer-Arendt, Kevin G. Pierce, Natalie G. Ahn, William M. Old, Robin D. Knight, Joel Sevinsky, Alex M Mendoza, Lauren D. Aveline-Wolf, Michelle A. Emrick, Katheryn A. Resing, Micah Hamady, Mariah C. Ruth
Publikováno v:
Journal of Proteome Research. 5:709-719
An important strategy for "shotgun proteomics" profiling involves solution proteolysis of proteins, followed by peptide separation using multidimensional liquid chromatography and automated sequencing by mass spectrometry (LC-MS/MS). Several protocol
Autor:
William M. Old, Kevin G. Pierce, Alex M Mendoza, Steven Russell, Geoff R. Goehle, Karen R. Jonscher, Karen Meyer-Arendt, Joy L. Wattawa, Hiu T. Cheung, Katheryn A. Resing, Robin D. Knight, Natalie G. Ahn, Lauren D. Aveline-Wolf
Publikováno v:
Analytical Chemistry. 76:3556-3568
Identifying proteins in cell extracts by shotgun proteomics involves digesting the proteins, sequencing the resulting peptides by data-dependent mass spectrometry (MS/MS), and searching protein databases to identify the proteins from which the peptid
Autor:
Rebecca E. Schweppe, Natalie G. Ahn, Hong Wang, Carrie H. Croy, John B. Shabb, Robert A. Brown, Katheryn A. Resing, Eric S. Witze, William M. Old, Kasey L. Couts, Karen Meyer-Arendt, Elizabeth S. Litman, José Garcia Vivas Miranda, Chia-Yu Yen, Stephane Houel
Summary Melanoma and other cancers harbor oncogenic mutations in the protein kinase B-Raf, which leads to constitutive activation and dysregulation of MAP kinase signaling. In order to elucidate molecular determinants responsible for B-Raf control of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::682e746344b954af2bb232bebf5e7178
https://europepmc.org/articles/PMC2735263/
https://europepmc.org/articles/PMC2735263/
Autor:
Shaojun Sun, Lawrence Hunter, William M. Old, Karen Meyer-Arendt, Katheryn A. Resing, Brian Eichelberger, Rob Knight, Chia-Yu Yen, Natalie G. Ahn, Stephane Houel
Publikováno v:
Molecularcellular proteomics : MCP. 8(4)
Identifying peptides from mass spectrometric fragmentation data (MS/MS spectra) using search strategies that map protein sequences to spectra is computationally expensive. An alternative strategy uses direct spectrum-to-spectrum matching against a re
Autor:
Karen Meyer-Arendt, Chia-Yu Yen, Katheryn A. Resing, Alex M Mendoza, Steve Russell, Shaojun Sun, Natalie G. Ahn, Krzysztof J. Cios
Publikováno v:
Analytical chemistry. 78(4)
Correct identification of a peptide sequence from MS/MS data is still a challenging research problem, particularly in proteomic analyses of higher eukaryotes where protein databases are large. The scoring methods of search programs often generate cas
Autor:
Joel Sevinsky, Kevin G. Pierce, Lauren D. Aveline-Wolf, Katheryn A. Resing, William M. Old, Karen Meyer-Arendt, Alex M Mendoza, Natalie G. Ahn
Publikováno v:
Molecularcellular proteomics : MCP. 4(10)
Measurements of mass spectral peak intensities and spectral counts are promising methods for quantifying protein abundance changes in shotgun proteomic analyses. We describe Serac, software developed to evaluate the ability of each method to quantify