Zobrazeno 1 - 10
of 126
pro vyhledávání: '"Karen M Polizzi"'
Publikováno v:
PLoS ONE, Vol 7, Iss 4, p e34512 (2012)
The development of processes to produce biopharmaceuticals industrially is still largely empirical and relies on optimizing both medium formulation and cell line in a product-specific manner. Current small-scale (well plate-based) process development
Externí odkaz:
https://doaj.org/article/6fec35983937487f936a559a725da4fa
Publikováno v:
Engineering Biology, Vol 6, Iss 2-3, Pp 62-68 (2022)
Abstract In synthetic biology, biosensors are routinely coupled with a gene expression system for detecting small molecules and physical signals. We reveal a fluorescent complex, based on the interaction of an Escherichia coli double bond reductase (
Externí odkaz:
https://doaj.org/article/ca0717437d9b49178306015f883d97e3
Autor:
Lakshmeesha K. Nagappa, Wakana Sato, Farzana Alam, Kameshwari Chengan, Christopher M. Smales, Tobias Von Der Haar, Karen M. Polizzi, Katarzyna P. Adamala, Simon J. Moore
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)
Cell-free gene expression (CFE) systems are an attractive tool for engineering within synthetic biology and for industrial production of high-value recombinant proteins. CFE reactions require a cell extract, energy system, amino acids, and DNA, to ca
Externí odkaz:
https://doaj.org/article/918bd92301994b41802d8d3e7a95371a
Autor:
Simon J. Moore, Yonek B. Hleba, Sarah Bischoff, David Bell, Karen M. Polizzi, Paul S. Freemont
Publikováno v:
Microbial Cell Factories, Vol 20, Iss 1, Pp 1-11 (2021)
Abstract Background A key focus of synthetic biology is to develop microbial or cell-free based biobased routes to value-added chemicals such as fragrances. Originally, we developed the EcoFlex system, a Golden Gate toolkit, to study genes/pathways f
Externí odkaz:
https://doaj.org/article/a7517874ba71408894243205086980df
Autor:
F. Dean Keck, Karen M. Polizzi
Publikováno v:
Microbial Biotechnology, Vol 14, Iss 1, Pp 26-30 (2021)
Externí odkaz:
https://doaj.org/article/21659a3fe3944ee5a4d3b5ebea8ee3b9
Publikováno v:
Synthetic and Systems Biotechnology, Vol 5, Iss 3, Pp 137-144 (2020)
A renaissance in cell-free protein synthesis (CFPS) is underway, enabled by the acceleration and adoption of synthetic biology methods. CFPS has emerged as a powerful platform technology for synthetic gene network design, biosensing and on-demand bio
Externí odkaz:
https://doaj.org/article/2c14f66d13b440f7990cf46e39759377
Autor:
Chiara Heide, Gizem Buldum, Ignacio Moya-Ramirez, Oscar Ces, Cleo Kontoravdi, Karen M. Polizzi
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2021)
In this paper, we describe the stepwise development of a cell-free protein synthesis (CFPS) platform derived from cultured Chinese hamster ovary (CHO) cells. We provide a retrospective summary of the design challenges we faced, and the optimized meth
Externí odkaz:
https://doaj.org/article/f133ad6541094d9fb046fb7b424242ee
Autor:
Simon J. Moore, Yonek B. Hleba, Sarah Bischoff, David Bell, Karen M. Polizzi, Paul S. Freemont
Publikováno v:
Microbial Cell Factories, Vol 20, Iss 1, Pp 1-2 (2021)
Externí odkaz:
https://doaj.org/article/218aa91a2e9441c4a45554578d2b6ece
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
Virus-like particles (VLPs) are supramolecular protein assemblies with the potential for unique and exciting applications in synthetic biology and medicine. Despite the attention VLPs have gained thus far, considerable limitations still persist in th
Externí odkaz:
https://doaj.org/article/135577779d2848fda05dce69ca054282
Publikováno v:
Synthetic and Systems Biotechnology, Vol 3, Iss 1, Pp 64-75 (2018)
Adaptation allows organisms to maintain a constant internal environment, which is optimised for growth. The unfolded protein response (UPR) is an example of a feedback loop that maintains endoplasmic reticulum (ER) homeostasis, and is characteristic
Externí odkaz:
https://doaj.org/article/946be861930b44bb94f3bfa50fb457f3