Zobrazeno 1 - 10
of 124
pro vyhledávání: '"Kapil Bharti"'
Autor:
Sayan Ghosh, Ruchi Sharma, Sridhar Bammidi, Victoria Koontz, Mihir Nemani, Meysam Yazdankhah, Katarzyna M. Kedziora, Donna Beer Stolz, Callen T. Wallace, Cheng Yu-Wei, Jonathan Franks, Devika Bose, Peng Shang, Helena M. Ambrosino, James R. Dutton, Zhaohui Geng, Jair Montford, Jiwon Ryu, Dhivyaa Rajasundaram, Stacey Hose, José-Alain Sahel, Rosa Puertollano, Toren Finkel, J. Samuel Zigler, Yuri Sergeev, Simon C. Watkins, Eric S. Goetzman, Deborah A. Ferrington, Miguel Flores-Bellver, Kai Kaarniranta, Akrit Sodhi, Kapil Bharti, James T. Handa, Debasish Sinha
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-18 (2024)
Abstract Non-neovascular or dry age-related macular degeneration (AMD) is a multi-factorial disease with degeneration of the aging retinal-pigmented epithelium (RPE). Lysosomes play a crucial role in RPE health via phagocytosis and autophagy, which a
Externí odkaz:
https://doaj.org/article/9a768d2563fb4e4f839cd5a860fabad3
Autor:
Tea Soon Park, Rishabh Hirday, Russell Quinn, Sheela Panicker Jacob, Ricardo A. Feldman, Devika Bose, Ruchi Sharma, Kapil Bharti
Publikováno v:
STAR Protocols, Vol 5, Iss 1, Pp 102827- (2024)
Summary: Here, we present a protocol to differentiate induced pluripotent stem cell (iPSC) into adherent hematopoietic progenitors that release floating CD14+ CD45+ monocytes into the culture medium. We describe steps for iPSC expansion, embryoid bod
Externí odkaz:
https://doaj.org/article/47b0693c0a584adc9446cc892a7a2ebf
Autor:
Viola Kretschmer, Sandra Schneider, Peter Andreas Matthiessen, Dominik Reichert, Nathan Hotaling, Gunnar Glasßer, Ingo Lieberwirth, Kapil Bharti, Rossella De Cegli, Ivan Conte, Emeline F Nandrot, Helen Louise May-Simera
Publikováno v:
PLoS Biology, Vol 21, Iss 12, p e3002402 (2023)
Vision impairment places a serious burden on the aging society, affecting the lives of millions of people. Many retinal diseases are of genetic origin, of which over 50% are due to mutations in cilia-associated genes. Most research on retinal degener
Externí odkaz:
https://doaj.org/article/9298e4b010bc4863967b740324cfb661
Autor:
Ashley M. Fortress, Kiyoharu J. Miyagishima, Amberlynn A. Reed, Sally Temple, Dennis O. Clegg, Budd A. Tucker, Timothy A. Blenkinsop, George Harb, Thomas N. Greenwell, Tenneille E. Ludwig, Kapil Bharti
Publikováno v:
Stem Cell Research & Therapy, Vol 14, Iss 1, Pp 1-13 (2023)
Abstract National Eye Institute recently issued a new Strategic Plan outlining priority research areas for the next 5 years. Starting cell source for deriving stem cell lines is as an area with gaps and opportunities for making progress in regenerati
Externí odkaz:
https://doaj.org/article/b5b13c2ea0074af788766f71aeeb249c
Publikováno v:
npj Regenerative Medicine, Vol 7, Iss 1, Pp 1-8 (2022)
This report summarizes key issues contributing to the cost of preparing human pluripotent stem cell lines for use in cell therapy manufacturing based on discussion between stem cell banking experts from ten countries at a workshop session on ‘cost
Externí odkaz:
https://doaj.org/article/94bfb214688b477fba0d9a56df75ddbd
Autor:
Francesca Barone, Juan Amaral, Irina Bunea, Mitra Farnoodian, Rohan Gupta, Rishabh Gupta, Dara Baker, M. Joseph Phillips, Richard J. Blanch, Arvydas Maminishkis, David M. Gamm, Kapil Bharti
Publikováno v:
JCI Insight, Vol 8, Iss 11 (2023)
Over 30 million people worldwide suffer from untreatable vision loss and blindness associated with childhood-onset and age-related eye diseases caused by photoreceptor (PR), retinal pigment epithelium (RPE), and choriocapillaris (CC) degeneration. Re
Externí odkaz:
https://doaj.org/article/31aba2db034c4e20911854cd3b29f75e
Autor:
Tea Soon Park, Rishabh Hirday, Amir Ali, Roba Megersa, Rafael Villasmil, Eric Nguyen, Kapil Bharti
Publikováno v:
STAR Protocols, Vol 4, Iss 2, Pp 102292- (2023)
Summary: Here, we present a protocol for differentiating human-induced pluripotent stem cells into three distinct mesodermal cell types: vascular endothelial cells (ECs), pericytes, and fibroblasts. We describe steps for using monolayer serum-free di
Externí odkaz:
https://doaj.org/article/8995b0f06276462da44c56af04311282
Autor:
Kiyoharu J. Miyagishima, Ruchi Sharma, Malika Nimmagadda, Katharina Clore-Gronenborn, Zoya Qureshy, Davide Ortolan, Devika Bose, Mitra Farnoodian, Congxiao Zhang, Andrew Fausey, Yuri V. Sergeev, Mones Abu-Asab, Bokkyoo Jun, Khanh V. Do, Marie-Audrey Kautzman Guerin, Jorgelina Calandria, Aman George, Bin Guan, Qin Wan, Rachel C. Sharp, Catherine Cukras, Paul A. Sieving, Robert B. Hufnagel, Nicolas G. Bazan, Kathleen Boesze-Battaglia, Sheldon Miller, Kapil Bharti
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-16 (2021)
Miyagishima et al. investigate the pathological mechanisms underlying mutant CTRP5 function in late-onset retinal degeneration (L-ORD). With human iPSC-derived RPE cells, they demonstrate that in L-ORD iRPE, constitutive activation of AMPK disrupts c
Externí odkaz:
https://doaj.org/article/24727da70edd48558b39804aab74c898
Autor:
Ruchi Sharma, Aman George, Malika Nimmagadda, Davide Ortolan, Barbosa-Sabanero Karla, Zoya Qureshy, Devika Bose, Roba Dejene, Genqing Liang, Qin Wan, Justin Chang, Balendu Shekhar Jha, Omar Memon, Kiyoharu Joshua Miyagishima, Aaron Rising, Madhu Lal, Eric Hanson, Rebecca King, Mercedes Maria Campos, Marc Ferrer, Juan Amaral, David McGaughey, Kapil Bharti
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-18 (2021)
Age-related macular degeneration is characterized by lipid-rich drusen deposits underneath the retinal pigment epithelium (RPE). Here the authors report an in vitro iPSC-RPE model for AMD that recapitulates drusen and RPE atrophy, and identify two dr
Externí odkaz:
https://doaj.org/article/988dc6dedea34110a9215b08c61d924e
Publikováno v:
STAR Protocols, Vol 3, Iss 3, Pp 101582- (2022)
Summary: RPE tissues are derived from induced pluripotent stem cells (iPSCs) to model retinal diseases and as a replacement therapy for macular degeneration. Here, we developed a robust and efficient directed differentiation protocol to generate pure
Externí odkaz:
https://doaj.org/article/dcf4455fd75b48d5946582cba238db9e