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pro vyhledávání: '"Kao‐Lu Pan"'
Autor:
Kao-Lu Pan, 潘考祿
91
A simple and efficient method was used to inactivate specific genes in Escherichia coli for the production of various mutant strains and observation of their relevant phenotypes. The degP gene, encoding a periplasmic protease/chaperone of Deg
A simple and efficient method was used to inactivate specific genes in Escherichia coli for the production of various mutant strains and observation of their relevant phenotypes. The degP gene, encoding a periplasmic protease/chaperone of Deg
Externí odkaz:
http://ndltd.ncl.edu.tw/handle/96016346801710643991
Autor:
Ming Hua Hsu, Pinpin Lin, Huei Ju Liu, Kao Lu Pan, Wei Jan Huang, Mei Ya Shen, Hui Ling Lee, Chia Wei Cheng, Ming-Hsien Tsai
Publikováno v:
Chemical Research in Toxicology. 27:1707-1719
trans,trans-2,4-Decadienal (tt-DDE), a lipid peroxidation product of linolieic acid, is the most abundant aldehyde identified in cooking oil fumes and is readily detectable in food products as well as in restaurant emissions. Previously, we have repo
Publikováno v:
Biotechnology and Bioengineering. 108:2932-2940
Cytochrome P450 1A2 (CYP1A2) is an important member of cytochrome P450 involved in drug metabolism. In this study, a cell line, Huh7-1A2-I-E, with high expression level of CYP1A2 is established based on Huh7 cells. To achieve this, we constructed a r
Publikováno v:
Biotechnology and Bioengineering. 96:956-966
High-level expression of recombinant penicillin acylase (PAC) using the strong trc promoter system in Escherichia coli is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress
Autor:
Ming-Hsien Tsai, Pinpin Lin, Mei Ya Shen, Tze Ta Huang, Kao Lu Pan, Ying Min Cheng, Ann-Joy Cheng, Ya Chu Tang, Chia Huei Lee
Publikováno v:
Oncotarget
// Chia-Huei Lee 1 , Kao-Lu Pan 2 , Ya-Chu Tang 1 , Ming-Hsien Tsai 2 , Ann-Joy Cheng 3 , Mei-Ya Shen 2 , Ying-Min Cheng 1 , Tze-Ta Huang 4 , Pinpin Lin 2 1 National Institute of Cancer Research, National Health Research Institutes, Taipei, Taiwan 2
Diosgenin, a plant-derived sapogenin, exhibits antiviral activity in vitro against hepatitis C virus
Publikováno v:
Journal of natural products. 74(4)
Diosgenin (3β-hydroxy-5-spirostene, 1), a plant-derived sapogenin, is used as a dietary supplement. However, the biological effects of 1 related to viral replication remain unexplored. In this study, the effects of 1 on hepatitis C virus (HCV) repli
A cell culture system for the production of hepatitis C virus (HCV) whole virions has greatly accelerated studies of the virus life cycle and the discovery of anti-HCV agents. However, the quantification of the HCV titers in a whole-virus infection/r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa29e455522b8883a67545acbecff1b3
https://europepmc.org/articles/PMC2772346/
https://europepmc.org/articles/PMC2772346/
The well-known genetic information of Escherichia coli and successful applications of recombinant DNA technology make it possible for a variety of attempts with genetic engineering techniques to overproduce recombinant proteins. Upon performing the c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59624c755ffef66dff42385e60f48c2a
https://europepmc.org/articles/PMC154077/
https://europepmc.org/articles/PMC154077/
Publikováno v:
Journal of Bacteriology. May2003, Vol. 185 Issue 10, p3020. 11p. 2 Black and White Photographs, 1 Diagram, 1 Chart, 10 Graphs.
Publikováno v:
BMC Biotechnology, Vol 12, Iss 1, p 83 (2012)
BMC Biotechnology
BMC Biotechnology
Background There are few studies that have examined the potential of RNA inference (RNAi) to increase protein production in the baculovirus expression vector system (BEVS). Spodoptera frugiperda (fall armyworm) (Sf)-caspase-1-repressed stable cells e