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Akademický článek

SpCas9-HF1 enhances accuracy of cell cycle-dependent genome editing by increasing HDR efficiency, and by reducing off-target effects and indel rates

Autor: Daisuke Matsumoto, Erina Matsugi, Kanae Kishi, Yuto Inoue, Kiyomi Nigorikawa, Wataru Nomura

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 35, Iss 1, Pp 102124- (2024)

In genome editing, it is important to avoid off-target mutations so as to reduce unexpected side effects, especially for therapeutic applications. Recently, several high-fidelity versions of SpCas9 have been developed to reduce off-target mutations.

Externí odkaz: https://doaj.org/article/51d5c0e3ef8a44fca6e7053d0086f249

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Cas9‐Geminin and Cdt1‐fused anti‐CRISPR protein synergistically increase editing accuracy

Autor: Daisuke Matsumoto, Kanae Kishi, Erina Matsugi, Yuto Inoue, Kiyomi Nigorikawa, Wataru Nomura

Publikováno v: FEBS Letters. 597:985-994

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6e7936611952d2cdc5d52f317c43aaca
https://doi.org/10.1002/1873-3468.14608

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The past, present, and future of artificial zinc finger proteins: design strategies and chemical and biological applications

Autor: Shigeru Negi, Miki Imanishi, Mami Hamori, Yuka Kawahara-Nakagawa, Wataru Nomura, Kanae Kishi, Nobuhito Shibata, Yukio Sugiura

Publikováno v: JBIC Journal of Biological Inorganic Chemistry. 28:249-261

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::67d45efec5cd556edf61f775843d6e23
https://doi.org/10.1007/s00775-023-01991-6

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An ImageJ-based tool for three-dimensional registration between different types of microscopic images

Autor: Hiroshi Koyama, Kanae Kishi, Seiya Mikoshiba, Toshihiko Fujimori

Publikováno v: Development, growthdifferentiation.

Three-dimensional (3D) registration (i.e. alignment) between two microscopic images is substantially helpful to study tissues not adhere to substrates such as mouse embryos and organoids which are often 3-dimensionally rotated during imaging. However

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e364eb14a867ed639f6e45e2642b7f69
https://pubmed.ncbi.nlm.nih.gov/36576380

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Importance of two-dimensional cation clusters induced by protein folding in intrinsic intracellular membrane permeability

Autor: Shigeru Negi, Mami Hamori, Yuka Kawahara-Nakagawa, Miki Imanishi, Miku Kurehara, Chieri Kitada, Yuri Kawahito, Kanae Kishi, Takayuki Manabe, Nobuyuki Kawamura, Hiroaki Kitagishi, Masato Mashimo, Nobuhito Shibata, Yukio Sugiura

Publikováno v: RSC chemical biology. 3(8)

Two-dimensional cation clusters formed on the surface of proteins play an important role in their intracellular translocation.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32dbcf0d48e7649c7c108e514dee4a3d
https://pubmed.ncbi.nlm.nih.gov/35975000

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A chordate species lacking Nodal utilizes calcium oscillation and Bmp for left–right patterning

Autor: Momoko Hayashi, Takeshi A. Onuma, Hiroki Nishida, Fuki Gyoja, Kai Wang, Kanae Kishi

Publikováno v: Proc Natl Acad Sci U S A

Larvaceans are chordates with a tadpole-like morphology. In contrast to most chordates of which early embryonic morphology is bilaterally symmetric and the left–right (L–R) axis is specified by the Nodal pathway later on, invariant L–R asymmetr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a44fef8331381b0433d432a2b8eec25a
http://id.nii.ac.jp/1394/00001379/

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Cover Image, Volume 59, Issue 12

Autor: Kanae Kishi, Hiroshi Koyama, Sanae Oka, Azusa Kato, Moritoshi Sato, Toshihiko Fujimori

Publikováno v: genesis. 59

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3d228dc4384e8b1f0709ed0aee1340d2
https://doi.org/10.1002/dvg.23463

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Repetitive short‐pulsed illumination efficiently activates photoactivatable‐Cre as continuous illumination in embryonic stem cells and pre‐implantation embryos of transgenic mouse

Autor: Hiroshi Koyama, Sanae Oka, Moritoshi Sato, Kanae Kishi, Azusa Kato, Toshihiko Fujimori

Publikováno v: genesis. 59

The Cre-loxP system has been widely used for specific DNA recombination which induces gene inactivation or expression. Recently, photoactivatable-Cre (PA-Cre) proteins have been developed as a tool for spatiotemporal control of the enzymatic activity

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::659348904b989119fbbf58e379cd3fae
https://doi.org/10.1002/dvg.23457

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Patterning and morphogenesis of the intricate but stereotyped oikoplastic epidermis of the appendicularian, Oikopleura dioica

Autor: Kanae Kishi, Momoko Hayashi, Hiroki Nishida, Takeshi A. Onuma

Publikováno v: Developmental Biology. 428:245-257

Mechanisms for morphogenetic processes that generate complex patterns in a reproducible manner remain elusive. Live imaging provides a powerful tool to record cell behaviors. The appendicularian, Oikopleura dioica, is a planktonic tunicate that has a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1521612002cbf060916163036ff915a8
https://doi.org/10.1016/j.ydbio.2017.06.008

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Long-distance cell migration during larval development in the appendicularian, Oikopleura dioica

Autor: Hiroki Nishida, Takeshi A. Onuma, Kanae Kishi

Publikováno v: Developmental Biology. 395(2):299-306

The appendicularian, Oikopleura dioica, is a planktonic chordate. Its simple and transparent body, invariant cell lineages and short life cycle of 5 days make it a promising model organism for studies of chordate development. Here we describe the cel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d04a8555119898bb01b86821939d34db

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