SP3 Protocol for Proteomic Plant Sample Preparation Prior LC-MS/MS

Autor: Kamil Mikulášek, Hana Konečná, David Potěšil, Renata Holánková, Jan Havliš, Zbyněk Zdráhal

Publikováno v: Frontiers in Plant Science, Vol 12 (2021)

Quantitative protein extraction from biological samples, as well as contaminants removal before LC-MS/MS, is fundamental for the successful bottom-up proteomic analysis. Four sample preparation methods, including the filter-aided sample preparation (

Externí odkaz: https://doaj.org/article/93236b1d89bb486a9aeb9fd4ef89f52f

Content of 4(5)-methylimidazole, caffeine and chlorogenic acid in commercial coffee brands

Autor: Thi Thanh Dieu Phan, Miroslava Bittová, Kamil Mikulášek, Stanislav Kráčmar, Vlastimil Kubáň, Pavel Valášek, Blanka Svobodová

Publikováno v: Potravinarstvo, Vol 9, Iss 1, Pp 31-38 (2015)

Content of 4(5)-methylimidazole (4-MeI), caffeine and chlorogenic acid in commercial coffee brands were determined using high-performance liquid chromatography (HPLC) with UV DAD and MS detectors. Positive ion ESI mass spectra of the 4-MeI standard y

Externí odkaz: https://doaj.org/article/755d351a5d46474eb1e9c95c0c52a34b

Assessing the Toxicity of 17α-Ethinylestradiol in Rainbow Trout Using a 4-Day Transcriptomics Benchmark Dose (BMD) Embryo Assay

Autor: Bradley Park, Jessica Ewald, Doug Crump, David J. Schneider, David Potěšil, Markus Hecker, Jianguo Xia, Connor Burbridge, Niladri Basu, Kamran Shekh, Alper James G. Alcaraz, Zbyněk Zdráhal, Kamil Mikulášek

Publikováno v: Environmental sciencetechnology. 55(15)

There is an urgent demand for more efficient and ethical approaches in ecological risk assessment. Using 17α-ethinylestradiol (EE2) as a model compound, this study established an embryo benchmark dose (BMD) assay for rainbow trout (RBT; Oncorhynchus

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39c88d34bb75e6fb1f619ef52175664b
https://pubmed.ncbi.nlm.nih.gov/34292719

Development of a Comprehensive Toxicity Pathway Model for 17α-Ethinylestradiol in Early Life Stage Fathead Minnows (

Autor: Alper James G, Alcaraz, David, Potěšil, Kamil, Mikulášek, Derek, Green, Bradley, Park, Connor, Burbridge, Kerstin, Bluhm, Othman, Soufan, Taylor, Lane, Marek, Pipal, Markus, Brinkmann, Jianguo, Xia, Zbyněk, Zdráhal, David, Schneider, Doug, Crump, Niladri, Basu, Natacha, Hogan, Markus, Hecker

Publikováno v: Environmental sciencetechnology. 55(8)

There is increasing pressure to develop alternative ecotoxicological risk assessment approaches that do not rely on expensive, time-consuming, and ethically questionable live animal testing. This study aimed to develop a comprehensive early life stag

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::352cf649710508cfd3162e7a7ab29d44
https://pubmed.ncbi.nlm.nih.gov/33755441

The oligomeric states of elicitins affect the hypersensitive response and resistance in tobacco

Autor: Martina Zapletalová, Annick Chiltz, Zbyněk Zdráhal, Marek Petřivalský, Kamil Mikulášek, Nathalie Leborgne-Castel, Martin Solanský, Jan Lochman

Publikováno v: Journal of Experimental Botany
Journal of Experimental Botany, Oxford University Press (OUP), 2021, 72 (8), pp.3219-3234. ⟨10.1093/jxb/erab011⟩

Successful plant defence against microbial pathogens is based on early recognition and fast activation of inducible responses. Key mechanisms include detection of microbe-associated molecular patterns by membrane-localized pattern recognition recepto

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78df13e384ea4ae0cb704acb8a274bb9
https://pubmed.ncbi.nlm.nih.gov/33475728