GAPR-1 Interferes with Condensate Formation of Beclin 1 in Saccharomyces cerevisiae

Autor: Sirati, Nafiseh, Shen, Ziying, Olrichs, Nick K., Popova, Blagovesta, Verhoek, Iris C., Lagerwaard, Ilse M., Braus, Gerhard H., Kaloyanova, Dora V., Helms, J.Bernd

Publikováno v: In Journal of Molecular Biology 28 February 2023 435(4)

Dynamic and Reversible Aggregation of the Human CAP Superfamily Member GAPR-1 in Protein Inclusions in Saccharomyces cerevisiae

Autor: Sirati, Nafiseh, Popova, Blagovesta, Molenaar, Martijn R, Verhoek, Iris C, Braus, Gerhard H, Kaloyanova, Dora V, Helms, J Bernd, dB&C FR-RMSC RMSC, Veterinaire biochemie, dB&C FR-RMSC FR

Publikováno v: Journal of Molecular Biology, 433(19), 1. Academic Press Inc.

Many proteins that can assemble into higher order structures termed amyloids can also concentrate into cytoplasmic inclusions via liquid-liquid phase separation. Here, we study the assembly of human Golgi-Associated plant Pathogenesis Related protein

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::932c98db5aee4f5b61b2cbe1feba27c4
https://dspace.library.uu.nl/handle/1874/412929

Regulation of Functional Protein Aggregation by Multiple Factors: Implications for the Amyloidogenic Behavior of the CAP Superfamily Proteins

Autor: Sheng, Jie, Olrichs, Nick K, Gadella, Bart M, Kaloyanova, Dora V, Helms, J Bernd, LS Veterinaire biochemie, dB&C FR-RMSC FR, FAH klinische reproductie, Veterinaire biochemie, dES/dFAH FR, dB&C FR-RMSC RMSC

Publikováno v: International Journal of Molecular Sciences, Vol 21, Iss 6530, p 6530 (2020)
International Journal of Molecular Sciences, 21(18). MDPI AG
International Journal of Molecular Sciences

The idea that amyloid fibrils and other types of protein aggregates are toxic for cells has been challenged by the discovery of a variety of functional aggregates. However, an identification of crucial differences between pathological and functional

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4b8b2ce2e1ea5d8715d11cb05d1641a
https://doi.org/10.3390/ijms21186530

Zinc binding regulates amyloid-like aggregation of GAPR-1

Autor: Sheng, Jie, Olrichs, Nick K, Geerts, Willie J, Li, Xueyi, Rehman, Ashfaq Ur, Gadella, Barend M, Kaloyanova, Dora V, Helms, J Bernd, LS Veterinaire biochemie, dB&C FR-RMSC FR, Sub Cryo - EM, dES/dFAH FR, Sub Reproductie mannelijk, Sub Biologie van de mannelijke gameet, dB&C FR-RMSC RMSC, Dep Biochemie en Celbiologie

Publikováno v: Bioscience Reports, 39(2). Portland Press Ltd.
Bioscience Reports

Members of the CAP superfamily (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 proteins) are characterized by the presence of a CAP domain that is defined by four sequence motifs and a highly conserved tertiary structure. A c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3b0a52882c515515db88de25b698b24
https://dspace.library.uu.nl/handle/1874/377965

Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle

Autor: Kaloyanova, Dora, Vogels, Mijke, van Balkom, Bas W. M., Helms, J. Bernd

Publikováno v: Salmonella

Quantitative proteomics, based on stable isotope labeling by amino acids in cell culture (SILAC), can be used to identify host proteins involved in the intracellular interplay with pathogens. This method allows identification of proteins subject to d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6f187e8c3c8995d24a44323762a1ab9c
http://europepmc.org/articles/PMC7121999