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Autor:
Delilah Jewel, Rachel E. Kelemen, Rachel L. Huang, Zeyu Zhu, Bharathi Sundaresh, Xiaofu Cao, Kaitlin Malley, Zeyi Huang, Muhammad Pasha, Jon Anthony, Tim van Opijnen, Abhishek Chatterjee
Publikováno v:
Nature Methods. 20:95-103
Site-specific incorporation of unnatural amino acids (Uaas) in living cells relies on engineered aminoacyl-transfer RNA synthetase-tRNA pairs borrowed from a distant domain of life. Such heterologous suppressor tRNAs often have poor intrinsic activit