Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Kaho Kajiwara"'
Autor:
Yusei Matsuzaki, Wataru Aoki, Takumi Miyazaki, Shunsuke Aburaya, Yuta Ohtani, Kaho Kajiwara, Naoki Koike, Hiroyoshi Minakuchi, Natsuko Miura, Tetsuya Kadonosono, Mitsuyoshi Ueda
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Abstract Optimisation of protein binders relies on laborious screening processes. Investigation of sequence–function relationships of protein binders is particularly slow, since mutants are purified and evaluated individually. Here we developed pep
Externí odkaz:
https://doaj.org/article/6ca696081ad148fc8e2a41f6d690936f
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
Abstract Yeast cell surface display (YSD) has been used to engineer various proteins, including antibodies. Directed evolution, which subjects a gene to iterative rounds of mutagenesis, selection and amplification, is useful for protein engineering.
Externí odkaz:
https://doaj.org/article/3c96bce5a6a04f1d89df7768632d6aad
Publikováno v:
AMB Express, Vol 10, Iss 1, Pp 1-10 (2020)
Abstract Yeast surface display is a powerful technology used to isolate and engineer proteins to improve their activity, specificity, and stability. In this method, gene expression is regulated by promoters, and secretion efficiency is affected by se
Externí odkaz:
https://doaj.org/article/17a3fdb26fba48e7b7feb74d89425c40
Autor:
Kana Miyamoto, Wataru Aoki, Yuta Ohtani, Natsuko Miura, Shunsuke Aburaya, Yusei Matsuzaki, Kaho Kajiwara, Yoshinori Kitagawa, Mitsuyoshi Ueda
Publikováno v:
PLoS ONE, Vol 14, Iss 4, p e0215993 (2019)
Measuring binding properties of binders (e.g., antibodies) is essential for developing useful experimental reagents, diagnostics, and pharmaceuticals. Display technologies can evaluate a large number of binders in a high-throughput manner, but the im
Externí odkaz:
https://doaj.org/article/c0de412af4f444c3a965f543cc618835
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2446
Single-domain antibodies (sdAbs) are binders that consist of a single immunoglobulin domain. SdAbs have gained importance as therapeutics, diagnostic reagents, and research tools. Functional sdAbs are commonly produced in Escherichia coli, which is a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e710ce053284e5fdea75d1115ed56127
https://pubmed.ncbi.nlm.nih.gov/35157274
https://pubmed.ncbi.nlm.nih.gov/35157274
Publikováno v:
Methods in Molecular Biology ISBN: 9781071620748
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1b9dcefdef003cb00e7e6d5337fd920f
https://doi.org/10.1007/978-1-0716-2075-5_9
https://doi.org/10.1007/978-1-0716-2075-5_9
Autor:
Shunsuke Aburaya, Yuta Ohtani, Wataru Aoki, Takumi Miyazaki, Naoki Koike, Yusei Matsuzaki, Hiroyoshi Minakuchi, Tetsuya Kadonosono, Mitsuyoshi Ueda, Kaho Kajiwara, Natsuko Miura
Publikováno v:
Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Optimisation of protein binders relies on laborious screening processes. Investigation of sequence–function relationships of protein binders is particularly slow, since mutants are purified and evaluated individually. Here we developed peptide barc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c72e48ce77db53e4d4c54ccc90ec0be9
https://pubmed.ncbi.nlm.nih.gov/34728738
https://pubmed.ncbi.nlm.nih.gov/34728738
Publikováno v:
The FASEB Journal. 35
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5ebae11da95f9f9734595426c5a636d7
https://doi.org/10.1096/fasebj.2021.35.s1.01554
https://doi.org/10.1096/fasebj.2021.35.s1.01554
Publikováno v:
Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
Yeast cell surface display (YSD) has been used to engineer various proteins, including antibodies. Directed evolution, which subjects a gene to iterative rounds of mutagenesis, selection and amplification, is useful for protein engineering. In vivo c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ce1a50e892a86442e28dd34f29baa3c
https://pubmed.ncbi.nlm.nih.gov/34040114
https://pubmed.ncbi.nlm.nih.gov/34040114
Publikováno v:
AMB Express, Vol 10, Iss 1, Pp 1-10 (2020)
AMB Express
AMB Express
Yeast surface display is a powerful technology used to isolate and engineer proteins to improve their activity, specificity, and stability. In this method, gene expression is regulated by promoters, and secretion efficiency is affected by secretion s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ceb6b95f8be196f70cded4d6d2aac7b5
https://doi.org/10.1186/s13568-020-00983-y
https://doi.org/10.1186/s13568-020-00983-y