Quantitative Microdialysis: Experimental Protocol and Software for Small Molecule Protein Affinity Determination and for Exclusion of Compounds with Poor Physicochemical Properties

Autor: Steven Shave, Nhan T. Pham, Connor B. Śmieja, Manfred Auer

Publikováno v: Methods and Protocols, Vol 3, Iss 3, p 55 (2020)

Quantitative microdialysis is a traditional biophysical affinity determination technique. In the development of the detailed experimental protocol presented, we used commercially available equipment, rapid equilibrium dialysis (RED) devices (ThermoFi

Externí odkaz: https://doaj.org/article/584297e7854f49fb8c08ddd461ed5cf2

Quantitative microdialysis:Experimental protocol and software for small molecule protein affinity determination and for exclusion of compounds with poor physicochemical properties

Autor: Nhan T. Pham, Steven Shave, Manfred Auer, Connor B. Śmieja

Publikováno v: Shave, S, Pham, N T, Smieja, C & Auer, M 2020, ' Quantitative microdialysis : Experimental protocol and software for small molecule protein affinity determination and for exclusion of compounds with poor physicochemical properties ', Methods and Protocols, vol. 3, no. 55, pp. 55 . https://doi.org/10.3390/mps3030055
Methods and Protocols, Vol 3, Iss 55, p 55 (2020)
Methods and Protocols

Quantitative microdialysis is a traditional biophysical affinity determination technique.In the development of the detailed experimental protocol presented, we used commercially availableequipment, rapid equilibrium dialysis (RED) devices (ThermoFish

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4e83df7c0173fdb67e868f5d0ef8b49
https://www.pure.ed.ac.uk/ws/files/163587822/mps_03_00055.pdf

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Native ESI Mass Spectrometry Can Help to Avoid Wrong Interpretations from Isothermal Titration Calorimetry in Difficult Situations

Autor: Eric Ennifar, Gilles Guichard, Philippe Wolff, Dominique Burnouf, Guillaume Bec, Philippe Dumas, Cyrielle Da Veiga

Publikováno v: Journal of The American Society for Mass Spectrometry
Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), 2017, 28 (2), pp.347-357. ⟨10.1007/s13361-016-1534-6⟩
Journal of the American Society for Mass Spectrometry

We studied by native ESI-MS the binding of various DNA-polymerase-derived peptides onto DNA-polymerase processivity rings from Escherichia coli, Pseudomonas aeruginosa, and Mycobacterium tuberculosis. These homodimeric rings present two equivalent sp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99b5792c55ac23cd99ee1eb392559bee
https://hal.archives-ouvertes.fr/hal-02171327

Ligand- and drug-binding studies of membrane proteins revealed through circular dichroism spectroscopy

Autor: Simon G. Patching, Mary K. Phillips-Jones, Rohanah Hussain, Giuliano Siligardi

Publikováno v: Biochimica et Biophysica Acta:Biomembranes
Biochimica et Biophysica Acta:Biomembranes, Elsevier, 2014, 1838 (1), pp.34-42. ⟨10.1016/j.bbamem.2013.06.019⟩

A great number of membrane proteins have proven difficult to crystallise for use in X-ray crystallographic structural determination or too complex for NMR structural studies. Circular dichroism (CD) is a fast and relatively easy spectroscopic techniq

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cf80797dd4d9e11942f1cc019a23f2e
https://pubmed.ncbi.nlm.nih.gov/23811229