Zobrazeno 1 - 10
of 46
pro vyhledávání: '"K.M. Rajkowski"'
Autor:
Nguyen Le Trang, Marie-Françoise Moreau, Solange de Lauzon, K.M. Rajkowski, N. Cittanova, Bernard Desfosses
Publikováno v:
Hybridoma. 9:481-491
The immunization procedure and immunogen characteristics required to optimize the production of anti-steroid monoclonal antibodies have been studied. Five different estradiol-bovine serum albumin conjugates were tested for immunizing mice, as were tw
Autor:
K.M. Rajkowski
Publikováno v:
Biochemical Pharmacology. 39:895-900
A theoretical evaluation of existing and new graphical procedures for estimating the intrinsic molar fluorescence (psi B) of a protein-bound drug has been undertaken. The results do not concord with a recent proposition that psi B should be obtained
Publikováno v:
The Journal of steroid biochemistry and molecular biology. 48(2-3)
Quantitative understanding of steroid hormone transport and receptor-mediated action requires knowledge of the bonding forces involved in each steroid-protein complex and the effects of a biological environment on these forces. An approach to these p
Autor:
Françoise Hervé, Lawrence T. Wong, N. Cittanova, Carleton J.C. Hsia, Myriam Gentin, K.M. Rajkowski
Publikováno v:
Journal of steroid biochemistry. 36(4)
Rat fetal serum alpha 1-fetoprotein (AFP), a heterogeneous glycoprotein, binds estrogens with high affinity but at a fractional number of sites even after treatment with charcoal (n = 0.6), which may mean 60% of the protein has 1 site and the remaind
Publikováno v:
Analytical Biochemistry. 159:179-186
A series of fluorescent disulfononaphthalimide (lucifer yellow) derivatives of testosterone and estriol have been synthesized: testosterone-3- and estriol-6-ureidooxime-lucifer yellow, testosterone-3-and estriol-6-(amidoureido)methoxyoxime lucifer ye
Autor:
G.D. Broadhead, K.M. Rajkowski
Publikováno v:
Journal of Chromatography A. 89:374-379
Publikováno v:
Steroids. 29:701-713
The formation of a horseradish peroxidase-testosterone conjugate for the enzyme-linked immunoassay of testosterone was investigated, using tritiated testosterone to follow the reaction. The formation of testosterone-3-(carboxymethyl) oxime-peroxidase
Publikováno v:
Clinica Chimica Acta. 164:71-82
The development of non-competitive microtitre plate enzyme-linked immunosorbent assays for steroids was investigated using immobilised steroid as immunosorbent and enzyme-labelled second antibodies. An assay for testosterone with polyclonal anti-test
Autor:
N. Cittanova, K.M. Rajkowski
Publikováno v:
Journal of Steroid Biochemistry. 14:861-866
The coupling of oestrone-3-glucuronide, oestriol-16α-glucuronide and pregnanediol-3α-glucuronide with alcohol dehydrogenase, glucose-6-phosphate dehydrogenase, urease (two preparations) and horseradish peroxidase has been investigated. Two carbodii
Publikováno v:
Computers and Biomedical Research. 20:324-332
A method is described for calculating the degree of competition for binding between two ligands which are bound at any number of site classes on a binding protein from a generalization of the equilibrium competitive binding equations, the protein's b