Výsledky vyhledávání - "K.J. Leenhouts"

Immunogenicity of a malaria parasite antigen displayed by Lactococcus lactis in oral immunisations

Autor: Jan Kok, S Yasawardena, Gerhardus Venema, K.J. Leenhouts, Aldert Zomer, Ranjan Ramasamy

Publikováno v: Vaccine
Vaccine, 24(18), 3900-3908. ELSEVIER SCI LTD

A putative protective protein from Plasmodium falciparum merozoites, MSA2, was expressed in two different ways on the cell surface of the Gram-positive food-grade bacterium, Lactococcus lactis. The first display format exploits an LPXTG-type anchorin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::499cd4895a448350c20fd03e4063ef53
http://europepmc.org/articles/PMC7115539

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A general system for generating unlabelled gene replacements in bacterial chromosomes

Autor: Girbe Buist, Albert Bolhuis, J. A. K. W. Kiel, M. Dabrowska, K.J. Leenhouts, Jan Kok, Gerard Venema, A M ten Berge, Igor Mierau

Publikováno v: Molecular and General Genetics MGG, 253(1-2), 217-224

A general system is described that facilitates gene replacements such that the recombinant strains are not labelled with antibiotic resistance genes. The method is based on the conditional replication of derivatives of the lactococcal plasmid pWV01,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::504203571db7748a891ea2528fc90c69
https://research.rug.nl/en/publications/e91ec93c-8501-402e-a6ba-7a53fa464988

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Mode of action of LciA, the lactococcin A immunity protein

Autor: K.J. Leenhouts, Alfred J. Haandrikman, Gerhardus Venema, T. Abee, K. Venema, de Louis Leij, R.E. Haverkort, Jan Kok

Publikováno v: Molecular Microbiology 14 (1994)
Molecular Microbiology, 14, 521-532
Molecular Microbiology, 14(3), 521-532. Wiley

Monoclonal antibodies were raised against a fusion between the Escherichia coli maltose-binding protein and LciA, the immunity protein that protects Lactococcus lactis against the effects of the bacteriocin lactococcin A. One of the antibodies direct

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d26d118fcbbee93349a9e4567d7c724a
https://doi.org/10.1111/j.1365-2958.1994.tb02186.x

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Lactococcal plasmid pWV01 as an integration vector for lactococci

Autor: Jan Kok, K.J. Leenhouts, Gerhardus Venema

Publikováno v: Applied and Environmental Microbiology. 57:2562-2567

A Bacillus subtilis strain was constructed that contained the repA gene of the lactococcal plasmid pWVO1 in its chromosome. This strain was used to construct the pWVO1-based integration vector pINT1, which lacked the repA gene. The 3.6-kb plasmid pIN

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3cbe50faad7e186eab50f9e3ae67de6
https://doi.org/10.1128/aem.57.9.2562-2567.1991

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Identification of a sodium chloride-regulated promoter in Lactococcus lactis by single-copy chromosomal fusion with a reporter gene

Autor: Gerard Venema, K.J. Leenhouts, Jan Willem Sanders, Jan Kok

Publikováno v: Molecular and General Genetics MGG, 257(6), 681-685

An integration vector, pORI13, was developed to screen in Lactococcus lactis for expression signals induced by changes in the environment and to assay transcriptional activity of genes in single copy. The plasmid carries a promoterless Escherichia co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e49298698b5f2268636a6848ff9e8348
https://pubmed.ncbi.nlm.nih.gov/9604892

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Construction of a food-grade multiple-copy integration system for Lactococcus lactis

Autor: Gerhardus Venema, Albert Bolhuis, Jan Kok, K.J. Leenhouts

Publikováno v: Applied Microbiology and Biotechnology, 49(4), 417-423. SPRINGER

A food-grade vector system was developed that allows stable integration of multiple plasmid copies in the chromosome of Lactococcus lactis. The vector consists of the plus origin of replication (Ori(+)) of the lactococcal plasmid pWV01, the sucrose g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55984da5a22bc3476930f0f44d6dccc7
https://research.rug.nl/en/publications/627d86ea-2076-418a-ad10-1e0e9c0e19eb

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Cloning and molecular analysis of the dihydrofolate reductase gene from Lactococcus lactis

Autor: K Leszczyńska, K.J. Leenhouts, Albert Bolhuis, Gerhardus Venema, P Cegłowski

Publikováno v: Europe PubMed Central

The Lactococcus lactis gene encoding trimethoprim resistance has been cloned and expressed in Escherichia coli and Bacillus subtilis. Several lines of evidence indicate that the cloned gene encodes dihydrofolate reductase (DHFR). (i) It fully complem

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::646f40e8884b05c9ec033fb17768ad26
https://europepmc.org/articles/PMC167319/

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Production of secreted guar α-galactosidase by Lactococcus lactis

Autor: Albert Bolhuis, K.J. Leenhouts, A. Ledeboer, Gerhardus Venema, Jan Kok

Publikováno v: Applied Microbiology and Biotechnology, 44(1). SPRINGER

A plant α-galactosidase gene was inserted in the expression vector pGKV259. The resulting plasmid pGAL2 consisted of the replication functions of the broad-host-range lactococcal plasmid pWV01, the lactococcal promoter P59, and the DNA sequences enc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::746847db929330c785e91c8b532d9909
https://research.rug.nl/en/publications/dc3c7416-3f23-4258-9cff-e8c9efef9e16

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Expression of a chitinase gene from Serratia marcescens in Lactococcus lactis and Lactobacillus plantarum

Autor: Alfred J. Haandrikman, K.J. Leenhouts, Gerhardus Venema, M B Brurberg, Ingolf F. Nes

Publikováno v: Applied microbiology and biotechnology. 42(1)

A chitinase gene from the Gram-negative bacterium Serratia marcescens BJL200 was cloned in Lactococcus lactis subsp. lactis MG1363 and in the silage inoculum strain Lactobacillus plantarum E19b. The chitinase gene was expressed as an active enzyme at

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c910f9cc46a8ec9b1d8ae1c27a25e64
https://pubmed.ncbi.nlm.nih.gov/7765812

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Mode of Action of Lactococcin B, a Thiol-Activated Bacteriocin from Lactococcus lactis

Autor: K.J. Leenhouts, Alfred J. Haandrikman, Jan Kok, Wn Konings, T Abee, Gerhardus Venema, K. Venema

Publikováno v: Applied and environmental microbiology, 59(4), 1041-1048. AMER SOC MICROBIOLOGY
Applied and Environmental Microbiology 59 (1993) 4
Applied and Environmental Microbiology, 59(4), 1041-1048

Lactococcin B (LcnB) is a small, hydrophobic, positively charged bacteriocin produced by Lactococcus lactis subsp. cremoris 9B4. Purified LcnB has a bactericidal effect on sensitive L. lactis cells by dissipating the proton motive force and causing l

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8edf60a17195be1f305075d6f71c5cb
https://research.rug.nl/en/publications/a9ca33b0-8635-4d1d-ba17-5467ee7bdbcb

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