Výsledky vyhledávání

Intracellular folding pathway of human chorionic gonadotropin beta subunit

Autor: Jeffrey R. Huth, F. Perini, Raymond W. Ruddon, K Mountjoy

Publikováno v: Journal of Biological Chemistry. 267:8870-8879

Few experimental models have been used to investigate how proteins fold inside a cell. Using the formation of disulfide bonds as an index of conformational changes during protein folding, we have developed a unique system to determine the intracellul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0c061927200d2ed0293625612155a501
https://doi.org/10.1016/s0021-9258(19)50361-1

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Disruption of active site interactions with pyridoxal 5'-phosphate and substrates by conservative replacements in the glycine-rich loop of Escherichia coli D-serine dehydratase

Autor: Christopher L. Kojiro, M Marceau, S D Lewis, Jules A. Shafer, K Mountjoy

Publikováno v: Journal of Biological Chemistry. 265:20421-20429

We have used site-directed mutagenesis to examine the function of three putative active site residues (C278, G279, and G281) of the vitamin B6 enzyme D-serine dehydratase. These residues lie in or adjacent to a conserved glycine-rich loop that is kno

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::95f15b27394e7e1724ca598838f42f2e
https://doi.org/10.1016/s0021-9258(17)30521-5

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Role of disulfide bond formation in the folding of human chorionic gonadotropin beta subunit into an alpha beta dimer assembly-competent form

Autor: K Mountjoy, Fulvio Perini, Jean Saccuzzo Beebe, Raymond W. Ruddon, Raymond F. Krzesicki

Publikováno v: Journal of Biological Chemistry. 265:312-317

The malignant trophoblastic cell line JAR was used as a model system to study protein folding in intact cells. We have used this model previously to identify conformational intermediates in the production of an assembly-competent form of the human ch

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::80219fc67a841c6087806e1eff14d335
https://doi.org/10.1016/s0021-9258(19)40231-7

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Amino acid composition, immunoreactivity, sequence analysis, and function of bovine hippocampal metallothionein isoforms

Autor: K. Mountjoy, J. S. Garvey, Manuchair Ebadi, F. Perini

Publikováno v: Journal of neurochemistry. 66(5)

The high concentration of zinc in the hippocampal mossy fiber axon boutons is localized in the vesicles and is mobilized by exocytosis of the zinc-laden vesicles. Because "free" zinc in excess is a neurotoxic substance inhibiting an extensive number

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::347eaa22af0237e07b2c8305706adf74
https://pubmed.ncbi.nlm.nih.gov/8780044

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The asparagine-linked oligosaccharides of the human chorionic gonadotropin beta subunit facilitate correct disulfide bond pairing

Autor: Martin M. Matzuk, Raymond W. Ruddon, Weijun Feng, K Mountjoy, Irving Boime, Elliott Bedows

Publikováno v: The Journal of biological chemistry. 270(20)

The role of asparagine (N)-linked oligosaccharide chains in intracellular folding of the human chorionic gonadotropin (hCG)-beta subunit was determined by examining the kinetics of folding in Chinese hamster ovary (CHO) cells transfected with wild-ty

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2b91000b11f09f1d143387954551e91
https://pubmed.ncbi.nlm.nih.gov/7538125

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Influence of acceptor substrate primary amino acid sequence on the activity of human UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferase. Studies with the MUC1 tandem repeat

Autor: I, Nishimori, N R, Johnson, S D, Sanderson, F, Perini, K, Mountjoy, R L, Cerny, M L, Gross, M A, Hollingsworth

Publikováno v: The Journal of biological chemistry. 269(23)

Synthetic peptides (30 and 20 residues long) corresponding to the native MUC1 tandem repeat sequence (20 residues long) were glycosylated in vitro using UDP-[3H]GalNAc and lysates from the human breast tumor cell line MCF7. Purified glycopeptides wer

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1d348932ff39075ebeddc7a274540d9e
https://pubmed.ncbi.nlm.nih.gov/8206912

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Folding of the β-Subunit of hCG and Its Role in Assembly of the α-β Heterodimer

Autor: Fulvio Perini, Jeffrey R. Huth, Elliott Bedows, K Mountjoy, Raymond W. Ruddon

Publikováno v: Glycoprotein Hormones ISBN: 9781461383888

It has been known for some time that the assembly of the α- and β-subunits of human chorionic gonadotropin (hCG) within cells that produce it is not totally efficient. As a result, uncombined α- and β-subunits, as well as α-β heterodimer, are s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f9d59a8e4fc0b7a22c82e7d07737f523
https://doi.org/10.1007/978-1-4613-8386-4_13

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Domain-dependent protein folding is indicated by the intracellular kinetics of disulfide bond formation of human chorionic gonadotropin beta subunit

Autor: J R, Huth, K, Mountjoy, F, Perini, E, Bedows, R W, Ruddon

Publikováno v: The Journal of biological chemistry. 267(30)

We have measured the intracellular rates of formation of the six disulfide bonds in the human chorionic gonadotropin beta subunit (hCG-beta) to determine whether the folding pathway of this molecule can be described by a simple sequential model. If s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d96bede2eedf1592b12cf2e2d66944bb
https://pubmed.ncbi.nlm.nih.gov/1400452

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