Zobrazeno 1 - 10
of 30
pro vyhledávání: '"K. H. Kroner"'
Publikováno v:
Biotechnology and Bioengineering. 104:217-239
Publikováno v:
Journal of Chemical Technology and Biotechnology. 32:130-137
Details of the process development and a flow diagram for the isolation of formate dehydrogenase from Candida boidinii are presented. Formate dehydrogenase was isolated from 30–50 kg of wet cells of C. boidinii to produce a technical-grade enzyme,
Publikováno v:
ChemInform. 23
Publikováno v:
Biotechnology Techniques. 5:199-204
Binding capacities for purified malate dehydrogenase from pig heart and malate dehydrogenase from Escherichia coli were determined for different gel matrices and a Cibacron Blue modified membrane. During batch adsorption the membrane has nearly the s
Autor:
J H, Vogel, K H, Kroner
Publikováno v:
Biotechnology and bioengineering. 63(6)
A novel rotary microfiltration technique specifically suited for the separation of animal cells has been developed. The concept allows the independent adjustment of wall shear stress, transmembrane pressure, and residence time, allowing straightforwa
Autor:
J. H. Vogel, K.-H. Kroner
Publikováno v:
Animal Cell Technology ISBN: 9789401062732
Dynamic microfiltration techniques are capable of yielding particle-free supernatants out of cell culture broth, which is often a pre-requisite for subsequent purification procedures. For controlling performance it is essential to generate sufficient
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c93743ccff15c25f114da327877ece7a
https://doi.org/10.1007/978-94-011-5404-8_65
https://doi.org/10.1007/978-94-011-5404-8_65
Publikováno v:
Animal Cell Technology ISBN: 9789401062732
Immunoaffinity chromatography is an interesting feature for the direct capture of pharmaceutical proteins out of cell culture broths, which can lead to improved production schemes. A prerequisite for the development of such a capture step is the choi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5dda89b3ce9367cd1837b72b0acf570f
https://doi.org/10.1007/978-94-011-5404-8_35
https://doi.org/10.1007/978-94-011-5404-8_35
Autor:
K. H. Kroner
Publikováno v:
Membrane Processes in Separation and Purification ISBN: 9789048144235
The application of affmity membranes in the downstream processing of proteins is of increasing interest. So far, affmity membranes have mainly been used in chromatography-analogous applications. Beside this, the use of such membranes could be of inte
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7a8195ac2b78060c93ec11c791cbc8ea
https://doi.org/10.1007/978-94-015-8340-4_6
https://doi.org/10.1007/978-94-015-8340-4_6
Autor:
K. H. Kroner
Publikováno v:
Membrane Processes in Separation and Purification ISBN: 9789048144235
The current status of the application of Cross-Flow Filtration in the downstream processing of bioproducts is reviewed. Applications, such as cell harvesting and the recovery of proteins from cellular debris are discussed and analyzed with respect to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2966005d425e6609b21f59f872e50675
https://doi.org/10.1007/978-94-015-8340-4_4
https://doi.org/10.1007/978-94-015-8340-4_4
Autor:
K. H. Kroner
Publikováno v:
Fresenius' Zeitschrift f�r Analytische Chemie. 329:718-725
The on-line determination of enzymes in biotechnical processes becomes an important factor with regard to process development and optimization. At present, most commonly enzymes are determined off-line in the laboratory after withdrawal of a separate