Zobrazeno 1 - 10
of 83
pro vyhledávání: '"K. H. Drexhage"'
Using two-photon excitation (TPE) at 700 nm as well as one-photon excitation (OPE) at 350 nm, we applied confocal fluorescence microscopy to detect single Coumarin-120 molecules in the solvents water and triacetin. To study the behavior of Coumarin-1
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69a0685f18ad4ab01f45aca7f888f30b
https://ora.ox.ac.uk/objects/uuid:7f8b7cdf-f105-49d2-8004-b4000e82e7cc
https://ora.ox.ac.uk/objects/uuid:7f8b7cdf-f105-49d2-8004-b4000e82e7cc
Publikováno v:
Chemical Physics Letters. 286:457-465
Using a confocal microscope we studied photon bursts from individual molecules (dye-labeled mononucleotides) flowing in a cone-shaped microcapillary with an inner diameter of 0.5 μm at the small end of the cone. The flow of the conjugates was establ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::af1ed85e1413145d72fef922a15b5b8f
https://doi.org/10.1016/s0009-2614(98)00096-7
https://doi.org/10.1016/s0009-2614(98)00096-7
Autor:
Klaus Mühlegger, C. Zander, Jürgen Wolfrum, R. Müller, Ulrike Lieberwirth, Florian Göbel, Jutta Arden-Jacob, K. H. Drexhage, Markus Sauer
Publikováno v:
Bioimaging. 6:14-24
We applied a short-pulse diode laser emitting at 640 nm with a repetition rate of 56 MHz in combination with a confocal microscope to study bursts of fluorescence photons from individual differently labeled mononucleotide molecules in water. Two newl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::814d95edf794b09be38e9664494ef8d7
https://doi.org/10.1002/1361-6374(199803)6:1<14::aid-bio3>3.0.co
https://doi.org/10.1002/1361-6374(199803)6:1<14::aid-bio3>3.0.co
Autor:
Markus Sauer, Ulrike Lieberwirth, Dirk P. Herten, R. Müller, Stefan Siebert, Jürgen Wolfrum, Michael Neumann, K. H. Drexhage, Andreas Schulz
Publikováno v:
Scopus-Elsevier
A new method is presented for automated one-lane four-dye DNA sequencing in capillary gel electrophoresis based on semiconductor technology and a special set of multiplex fluorescent dyes which exhibit similar absorption and emission spectra but diff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40e39cdd51496befcbe3e04d5ca8e0de
https://doi.org/10.1016/s0009-2614(97)01041-5
https://doi.org/10.1016/s0009-2614(97)01041-5
Publikováno v:
Scopus-Elsevier
The fluorescence bursts of individual antibody molecules BM-7 (IgG1) labeled with single dye molecules were detected and identified by the characteristic fluorescence lifetimes of the dyes Cy5 (1: 5n s) and JA169 (2: 7n s) directly in neat human seru
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cc2c8dc21effab5b9a11ed107968ad7
https://doi.org/10.1007/s003400050292
https://doi.org/10.1007/s003400050292
Time-resolved identification of single molecules in solution with a pulsed semiconductor diode laser
Autor:
C. Zander, K. H. Drexhage, M. Deimel, Stefan Siebert, G. Deltau, Markus Sauer, Jutta Arden-Jacob, N. J. Marx, D.-S. Ko, R. Müller, Jürgen Wolfrum
Publikováno v:
Chemical Physics Letters. 262:716-722
We used a confocal microscope to study bursts of fluorescence photons from single dye molecules excited at 638 nm by a short-pulsed diode laser with a repetition rate of 17 MHz. Four newly synthesized dyes (JA 167, DR 333, cyanorhodamine B and MR 121
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::05e80abd6a1e19b0077d20d9806ebbb9
https://doi.org/10.1016/s0009-2614(96)01147-5
https://doi.org/10.1016/s0009-2614(96)01147-5
Autor:
Karl Otto Greulich, C. Hoyer, K.-H. Drexhage, Eckhard Birch-Hirschfeld, J. Scheef, Holger Eickhoff
Publikováno v:
Journal of Biochemical and Biophysical Methods. 32:59-68
Three sets of partly overlapping octanucleotides are 5' labelled with derivates of the fluorescence dyes fluorescein-, coumarine- and rhodamine, respectively. Hybridisation conditions are determined, under which all octanucleotides hybridise correctl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c5b7c6aec57792c3b7d4298e766175f
https://doi.org/10.1016/0165-022x(95)00003-a
https://doi.org/10.1016/0165-022x(95)00003-a
Autor:
K. H. Drexhage, A. Fischer, Jutta Arden-Jacob, R. Müller, Jürgen Wolfrum, Stefan Seeger, M. Köllner
Publikováno v:
Chemical Physics Letters. 250:355-360
A concept taken from information theory is applied to ultrasensitive fluorescence detection and identification of molecules. The theoretical misidentification probability is compared to the experimental error probability. It is found that 350 photons
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a13470a2a1f0a8b89a00aad07be8b825
https://doi.org/10.1016/0009-2614(96)00031-0
https://doi.org/10.1016/0009-2614(96)00031-0
Autor:
Malte Köllner, K.-T. Han, K. H. Drexhage, Stefan Seeger, R. Müller, Jutta Arden-Jacob, Jürgen Wolfrum, Markus Sauer, G. Bachteler
Publikováno v:
Journal of Luminescence. 62:101-108
A detector for laser induced fluorescence based on laser diole technology was designed and sensitivity measurements with rhodamine 800 were performed. The detection limit is around 100 molecules in the detection volume. Using the laser diode in combi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::50d0963a2a8f45a93f1db555e57d91a9
https://doi.org/10.1016/0022-2313(94)90336-0
https://doi.org/10.1016/0022-2313(94)90336-0
Autor:
Jutta Arden-Jacob, R. Mu¨ller, M. Ko¨llner, Jürgen Wolfrum, Stefan Seeger, Markus Sauer, K. H. Drexhage, G. Bachteler, K.-T. Han
Publikováno v:
Journal of Luminescence. :511-514
A laser diode based detector for laser-induced fluorescence was designed. Sensitivity measurements with rhodamine 800 showed a detection limit of 100 molecules. Using time-resolved fluorescence measurements new fluorescent dyes, called multiplex dyes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b38a97ba92f84064a7342b64d34c7a87
https://doi.org/10.1016/0022-2313(94)90204-6
https://doi.org/10.1016/0022-2313(94)90204-6