Rapid assessment of cell viability of Lactobacillus delbrueckii subsp. bulgaricus by measurement of intracellular pH in individual cells using fluorescence ratio imaging microscopy

Autor: K. Björn Rechinger, Henrik Siegumfeldt

Publikováno v: International Journal of Food Microbiology. 75:53-60

The aim of this study was to investigate if the measurement of intracellular pH (pH i ) of individual cells by fluorescence ratio imaging microscopy (FRIM) could be utilized as a rapid method for determining the bacterial viability, using Lactobacill

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::737bc15474d214d12950bd60f03f7383
https://doi.org/10.1016/s0168-1605(01)00745-0

Noninvasive Measurement of Bacterial Intracellular pH on a Single-Cell Level with Green Fluorescent Protein and Fluorescence Ratio Imaging Microscopy

Autor: Mogens Jakobsen, Hanne Ingmer, Birgitte Bjørn Budde, Henrik Siegumfeldt, Katja N. Olsen, K. Björn Rechinger

Publikováno v: Applied and Environmental Microbiology. 68:4145-4147

We show that a pH-sensitive derivative of the green fluorescent protein, designated ratiometric GFP, can be used to measure intracellular pH (pH i ) in both gram-positive and gram-negative bacterial cells. In cells expressing ratiometric GFP, the exc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94542ab8b46695d3e9f0368e4c1b92d2
https://doi.org/10.1128/aem.68.8.4145-4147.2002

Dynamic changes of intracellular pH in individual lactic acid bacterium cells in response to a rapid drop in extracellular pH

Autor: Mogens Jakobsen, Henrik Siegumfeldt, K. Björn Rechinger

Publikováno v: University of Copenhagen

We describe the dynamics of changes in the intracellular pH (pH i ) values of a number of lactic acid bacteria in response to a rapid drop in the extracellular pH (pH ex ). Strains of Lactobacillus delbrueckii subsp. bulgaricus , Streptococcus thermo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8a9c20023d886ca30abe61a6dbadd65
https://pubmed.ncbi.nlm.nih.gov/10831407

High-level resistance to class IIa bacteriocins is associated with one general mechanism in Listeria monocytogenes

Autor: Yann Héchard, Anne Gravesen, Natalie Andersen, Manilduth Ramnath, John W. Hastings, K. Björn Rechinger, Susanne Knøchel, Lothar Jänsch

Publikováno v: ResearcherID
Europe PubMed Central

Class IIa bacteriocins may be used as natural food preservatives, yet resistance development in the target organisms is still poorly understood. In this study, the understanding of class IIa resistance development in Listeria monocytogenes is extende

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb15ab5a5acebf9bf885b06eb80afb5a
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000177529800014&KeyUID=WOS:000177529800014

Dynamic changes of intracellular pH in individual lactic acid bacterium cells in response to a rapid drop in extracellular pH.

Autor: Siegumfeldt H; Department of Dairy and Food Science, Food Microbiology, The Royal Veterinary and Agricultural University, 1958 Frederiksberg C, Denmark. hsi@kvl.dk, Björn Rechinger K, Jakobsen M

Publikováno v: Applied and environmental microbiology [Appl Environ Microbiol] 2000 Jun; Vol. 66 (6), pp. 2330-5.