Zobrazeno 1 - 10
of 10
pro vyhledávání: '"K. A. Musiychuk"'
Publikováno v:
Molecular Biology. 37:307-313
Bifunctional reporter proteins were constructed to combine Clostridium thermocellum lichenase (LicBM2) with Aequorea victoria green fluorescent protein (GFP) or with Escherichia coli β-glucuronidase (GUS). The major properties of the initial protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3b7cd6bd2a6449ea5a033307b088a360
https://doi.org/10.1023/a:1023310124842
https://doi.org/10.1023/a:1023310124842
Publikováno v:
Molecular Biology. 36:698-704
A new reporter system was developed to study the gene expression regulation in prokaryotic (Escherichia coli) and eukaryotic (Saccharomyces cerevisiae and mammalian) cells. The system was based on the modified bacterial lichenase gene (licBM2), which
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3779d1104f2cf21462d02c90c365e2be
https://doi.org/10.1023/a:1020631615748
https://doi.org/10.1023/a:1020631615748
Autor:
E. S. Piruzian, H. Alizadeh, I. V. Goldenkova, R. M. Abdeev, K. A. Musiychuk, L. G. Berdichivets
Publikováno v:
Russian Journal of Plant Physiology. 49:364-368
The expression of the bacterial gene for thermostable β-1,3-glucanase in transgenic tobacco plants was shown to induce substantial changes in plant morphogenetic potential, whereas the expression of β-1,3; 1,4-glucanase did not affect essentially p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::63ba95e2cefa8ae6a2d26fe65cdceee1
https://doi.org/10.1023/a:1015597103567
https://doi.org/10.1023/a:1015597103567
Autor:
I. V. Bobrysheva, E. S. Piruzian, I. V. Goldenkova, D. V. Glazkova, Chekhuta Ia, N. S. Kobets, K. A. Musiychuk, Arman Ip
Publikováno v:
Molecular Genetics and Genomics. 266:778-786
The coding region of the licB gene from Clostridium thermocellum was truncated at the 3' end. The modified lichenase encoded by the construct (LicBM2) retained the most important properties of the enzyme - its high activity and thermostability. LicBM
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c35e7fe8044f437d9a352d1896348374
https://doi.org/10.1007/s00438-001-0595-8
https://doi.org/10.1007/s00438-001-0595-8
Publikováno v:
Russian Journal of Plant Physiology. 49:524-529
The gene encoding xylose(glucose) isomerase (P00944, EC 5.3.1.5) in Escherichia coli was put under the control of the 35S CaMV promoter and transferred to Nicotiana tabacum L. plants using an Agrobacterium tumefaciens vector. Transgenic plants, which
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d96facb75878d21065cdffa3a8692546
https://doi.org/10.1023/a:1016320211336
https://doi.org/10.1023/a:1016320211336
Publikováno v:
Russian Journal of Genetics. 37:610-616
It is shown that bacterial genes for thermostable β-glucanases are expressed retaining their activity and substrate specificity. The leader peptide of the carrot extensin exerts effective secretion of the bacterial enzymes into the intercellular spa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f8396f414cf02e64d7edf04486c64dd0
https://doi.org/10.1023/a:1016613005650
https://doi.org/10.1023/a:1016613005650
Publikováno v:
Biochemistry (Moscow). 66:808-813
The main properties (pH and temperature range, stability, substrate specificity) of the modified cellulase CelE (endo-β-1,4-glucanase) from Clostridium thermocellum have been analyzed with the goal of its expression in plants. The modified enzyme is
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::37bcba946cc98635d08d708af761d26c
https://doi.org/10.1023/a:1010277115744
https://doi.org/10.1023/a:1010277115744
Publikováno v:
Biochemistry (Moscow). 65:1397-1402
Major properties (pH and temperature optimum, stability) of lichenase (b-1,3-1,4-glucanase) deletion variants from Clostridium thermocellum were comparatively studied. The deletion variant LicBM2 was used to create hybrid bifunctional proteins by fus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d0f6ca857c4a18ee867073270ceaeef4
https://doi.org/10.1023/a:1002804923384
https://doi.org/10.1023/a:1002804923384
Publikováno v:
Biochemistry. Biokhimiia. 66(7)
The main properties (pH and temperature range, stability, substrate specificity) of the modified cellulase CelE (endo-beta-1,4-glucanase) from Clostridium thermocellum have been analyzed with the goal of its expression in plants. The modified enzyme
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::ab91c584f6c3d076154ba3a9b6badb91
https://pubmed.ncbi.nlm.nih.gov/11563963
https://pubmed.ncbi.nlm.nih.gov/11563963
Publikováno v:
Biochemistry. Biokhimiia. 65(12)
Major properties (pH and temperature optimum, stability) of lichenase (beta-1,3-1,4-glucanase) deletion variants from Clostridium thermocellum were comparatively studied. The deletion variant LicBM2 was used to create hybrid bifunctional proteins by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::fca7c6ac22b665b71381d3f01df554b5
https://pubmed.ncbi.nlm.nih.gov/11173511
https://pubmed.ncbi.nlm.nih.gov/11173511