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pro vyhledávání: '"K Y, Jackson"'
Autor:
K Y Jackson, Richard W. Jenny
Publikováno v:
Clinical Chemistry. 39:76-81
Proficiency testing (PT) is pivotal in assessing laboratory qualifications for certification and licensure. PT is expected to typify routine assay performance and determine whether the laboratory is producing clinically useful test results. Conventio
Autor:
K Y Jackson, Richard W. Jenny
Publikováno v:
Clinical Chemistry. 38:496-500
The proposed rule for the Clinical Laboratory Improvement Amendments of 1988 (CLIA '88) describes uniform standards for the design of proficiency testing (PT) challenges and for the evaluation of laboratory performance. Successful performance on PT i
Autor:
R W, Jenny, K Y, Jackson
Publikováno v:
Clinical chemistry. 39(1)
Proficiency testing (PT) is pivotal in assessing laboratory qualifications for certification and licensure. PT is expected to typify routine assay performance and determine whether the laboratory is producing clinically useful test results. Conventio
Autor:
R W, Jenny, K Y, Jackson
Publikováno v:
Clinical chemistry. 38(4)
The proposed rule for the Clinical Laboratory Improvement Amendments of 1988 (CLIA '88) describes uniform standards for the design of proficiency testing (PT) challenges and for the evaluation of laboratory performance. Successful performance on PT i
Publikováno v:
Clinical Chemistry. 24:261-266
Optimal reaction conditions for assaying human lactate dehydrogenase pyruvate-to-lactate were determined for isoenzymes 1 and 5 at 25, 30, and 37 degrees C. Three of the nine different buffers examined--imidazole, triethanolamine, and N-tris(hydroxym
Publikováno v:
Clinical Chemistry. 23:200-204
The lactate-to-pyruvate reaction for serum lactate dehydrogenase (LD) is most frequently assayed in one of three buffers, pyrophosphate (PPi), tris(hydroxymethyl)amino-methane (Tris), or 2-amino-2-methyl-1-propanol (AMP). We described interconverting
Autor:
K Y Jackson, S N Buhl
Publikováno v:
Clinical Chemistry. 24:828-831
We report optimal conditions for assaying highly purified human lactate dehydrogenase isoenzymes with the lactate-to-pyruvate and pyruvate-to-lactate reactions, as they apply to human serum. Interconversion of results between reactions is not practic
Publikováno v:
Clinica Chimica Acta. 80:265-270
The kinetic constants of human lactate dehydrogenase 1 and 5 (L-lactate: NAD+ oxidoreductase, EC 1.1.1.27), assayed lactate-to-pyruvate increase with temperature. The reaction mechanism is ordered sequential as has been found with lactate dehydrogena
Publikováno v:
Clinical Chemistry. 22:1872-1875
Highly purified human lactate dehydrogenases I and V were assayed in 17 different buffers, at a variety of reaction pH's. Diethanolamine and 2-amino-2-methyl-1,3-propanediol provided the best measurements of the enzyme, assayed lactate-to-pyruvate. H
Publikováno v:
Clinical Chemistry. 27:1401-1409
An inactivator of alkaline phosphatase (EC 3.1.3.1) in 2-amino-2-methyl-1-propanol is demonstrated and characterized. This time-dependent inactivation results from chelation of enzyme-bound Zn2+; it is reversed by addition of Zn2+ and, to a lesser ex