Zobrazeno 1 - 10
of 63
pro vyhledávání: '"K T Tokuyasu"'
Publikováno v:
Proceedings of the National Academy of Sciences. 91:1878-1882
The role of cytoskeletal elements in the cellularization of syncytial Drosophila embryos is becoming evident; however, the distribution and role of organelles such as the Golgi complex, essential for membrane biogenesis, remain unknown. We have clone
Publikováno v:
Europe PubMed Central
Summary A specific type of premessenger RNP particle, Balbiani ring granules from the dipteran Chironomus tentans, was biochemically isolated and visualized in three dimensions with electron microscope tomography. The particles were prepared for elec
Autor:
K. T. Tokuyasu
Publikováno v:
ACTA HISTOCHEMICA ET CYTOCHEMICA. 25:143-146
When sections embedded in epoxy resin were conventionally stained with lead citrate and washed very briefly, about 1 sec, a very high staining intensity was attained. However, the staining intensity was quickly reduced by washing 10 min. When uranyl
Publikováno v:
Histochemistry. 95:123-136
A method is presented for increasing the contrast of cellular structures on ultrathin sections from tissues embedded in Lowicryl K4M. The method, designated UA/MC adsorption staining, is based on the uranyl acetate/methyl cellulose staining of thawed
Publikováno v:
The Journal of Cell Biology
The ultrastructural localization of three cytoskeletal proteins, alpha-actinin, tropomyosin, and vinculin, in the brush border of epithelial cells of chicken small intestine and the smooth muscle cells of chicken gizzard was studied by immunofluoresc
Publikováno v:
Journal of Cellular Physiology. 95:239-257
Experiments have been carried out on the capping by lectins and antibodies of surface receptors of mouse splenic T and B lymphocytes and other cells, in which the surface distribution of the lectin or antibody, and the intracellular distribution of m
Publikováno v:
The Journal of Cell Biology
Antibodies against chicken erythrocyte vimentin and gizzard desmin were affinity purified and then cross-absorbed with the heterologous antigen. They were used to study the in vivo distributions of these proteins in developing and mature myotubes by
Publikováno v:
Chromosoma. 83:593-617
The effects of spermatogenesis of a series of continguous non-overlapping Y-chromosome deficiencies were examined using both the light and electron microscope. The deficiencies were constructed by combining elements of different X-Y translocations; t
Publikováno v:
The Journal of Cell Biology
The distribution of the intermediate filament proteins vimentin and desmin in developing and mature myotubes in vivo was studied by single and double immunoelectron microscopic labeling of ultrathin frozen sections of iliotibialis muscle in 7-21-d-ol
Publikováno v:
The Journal of Cell Biology
The ultrastructural localization of the Ca2+ + Mg2+-dependent ATPase of sarcoplasmic reticulum in rat gracilis muscle was determined by indirect immunoferritin labeling of ultrathin frozen sections. Simultaneous visualization of ferritin particles an